Watanabe T, Aonuma H
Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, 060-0812, Japan.
Acta Biol Hung. 2012;63 Suppl 2:58-62. doi: 10.1556/ABiol.63.2012.Suppl.2.5.
Biogenic amine serotonin (5-HT) modulates various aspects of behaviors such as aggressive behavior and circadian behavior in the cricket. In our previous report, in order to elucidate the molecular basis of the cricket 5-HT system, we identified three genes involved in 5-HT biosynthesis, as well as four 5-HT receptor genes (5-HT1A, 5-HT1B, 5-HT2α, and 5-HT7) expressed in the brain of the field cricket Gryllus bimaculatus DeGeer [7]. In the present study, we identified Gryllus 5-HT2β gene, an additional 5-HT receptor gene expressed in the cricket brain, and examined its tissue-specific distribution and embryonic stage-dependent expression. Gryllus 5-HT2β gene was ubiquitously expressed in the all examined adult tissues, and was expressed during early embryonic development, as well as during later stages. This study suggests functional differences between two 5-HT2 receptors in the cricket.
生物胺5-羟色胺（5-HT）调节蟋蟀行为的各个方面，如攻击行为和昼夜节律行为。在我们之前的报告中，为了阐明蟋蟀5-HT系统的分子基础，我们鉴定了三个参与5-HT生物合成的基因，以及在双斑蟋蟀（Gryllus bimaculatus DeGeer）大脑中表达的四个5-HT受体基因（5-HT1A、5-HT1B、5-HT2α和5-HT7）[7]。在本研究中，我们鉴定了蟋蟀5-HT2β基因，这是另一个在蟋蟀大脑中表达的5-HT受体基因，并研究了其组织特异性分布和胚胎阶段依赖性表达。蟋蟀5-HT2β基因在所有检测的成年组织中普遍表达，并且在胚胎发育早期以及后期阶段均有表达。本研究表明蟋蟀中两种5-HT2受体之间存在功能差异。
