Meyering-Vos M, Müller A
University of Bayreuth, Bayreuth, Germany.
Insect Mol Biol. 2007 Aug;16(4):445-54. doi: 10.1111/j.1365-2583.2007.00737.x. Epub 2007 May 4.
The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Mediterranean field cricket Gryllus bimaculatus. The cDNA encodes two peptides flanked by endoproteolytic processing sites, designated GrybiSKI (QSDDYGHMRFG) and GrybiSKII (EPFDDYGHMRFG). The peptides include the characteristic amino acid Tyr, which is potentially sulphated, and a Gly, as a recognition site for amidation yeilding the common C-terminal amino acid sequence of the sulfakinin peptide family. RT-PCR studies indicate an expression of the gene restricted to the brain, with a constant level of expression throughout the last larval stage, but showing an age-dependent decrease of expression in adult females.
速激肽是多功能昆虫神经肽，与胃泌素/胆囊收缩素（CCK）肽家族具有序列相似性。在脊椎动物中，胃泌素和CCK肽参与消化和食物摄入的调节。在本研究中，从地中海田野蟋蟀双斑蟋中克隆并测序了速激肽cDNA。该cDNA编码两个由内切蛋白水解加工位点侧翼的肽，命名为GrybiSKI（QSDDYGHMRFG）和GrybiSKII（EPFDDYGHMRFG）。这些肽包括特征性氨基酸酪氨酸，其可能被硫酸化，以及一个甘氨酸，作为酰胺化的识别位点，产生速激肽肽家族常见的C末端氨基酸序列。逆转录-聚合酶链反应（RT-PCR）研究表明该基因的表达仅限于大脑，在最后一个幼虫阶段表达水平恒定，但在成年雌性中表达呈现年龄依赖性下降。
