STI Clinic, Infirmary, South Victoria University Hospital, Cork, Ireland.
Hum Vaccin Immunother. 2012 Jul;8(7):916-20. doi: 10.4161/hv.20122. Epub 2012 Jul 1.
The aim of this study was to determine the cervical genotype profile of females who presented to an STI Clinic with external genital warts (EGW); and to determine the potential vaccine coverage prior to the uptake of the HPV vaccines. Sixty-one cervical scrapings were taken from females aged 18-35 y who had external genital warts or a history of external genital warts. The resulting 50 samples that were positive for HPV-DNA were subjected to genotype identification. Forty-six of these samples had detectable genotypes by LIPA analysis and most (78%, 36/46) had multiple low risk (LR) and high risk (HR) genotypes on the cervix. Twenty-five of these samples (54%) had more than 1 HR genotype. Of the 36 patients who had any HR genotypes, 18 (50%) were identified to have the most oncogenic HPV genotypes, namely 16 and 18. Three of these samples had both 16 and 18 on the cervix. The presence of multiple HR genotypes on the majority of cervical samples from a self-referred population of females with EGW is presented. This study is of importance since persistent HR-HPV is the necessary risk factor in the development of precancerous and cancerous lesions of the cervix. Gardisil, the quadrivalent HPV vaccine would have been useful in the prevention of 28% (13/46) of these infections.
本研究旨在确定在性传播感染（STI）诊所就诊的患有生殖器外部疣（EGW）的女性的宫颈基因型谱；并确定 HPV 疫苗接种前的潜在疫苗覆盖率。对年龄在 18-35 岁之间患有生殖器外部疣或有生殖器外部疣病史的女性进行了 61 次宫颈刮片检查。从 HPV-DNA 阳性的 50 个样本中进行基因型鉴定。通过 LIPA 分析检测到 46 个具有可检测基因型的样本，其中大多数（78%，36/46）在宫颈上具有多个低危（LR）和高危（HR）基因型。这 46 个样本中有 25 个（54%）具有超过 1 种 HR 基因型。在 36 名有任何 HR 基因型的患者中，有 18 名（50%）被确定为具有最致癌 HPV 基因型，即 16 型和 18 型。其中 3 个样本在宫颈上同时存在 16 型和 18 型。来自有 EGW 的自我报告女性人群的大多数宫颈样本中存在多种 HR 基因型。由于持续存在 HR-HPV 是宫颈癌前病变和癌症发展的必要危险因素，因此本研究具有重要意义。Gardisil 四价 HPV 疫苗本可以预防这些感染中的 28%（13/46）。
