Department of Biomaterials, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500, HB, Nijmegen, The Netherlands.
J Tissue Eng Regen Med. 2014 Jun;8(6):473-82. doi: 10.1002/term.1546. Epub 2012 Jul 6.
The present study aimed to provide information on material degradation and subsequent alveolar bone formation, using composites consisting of calcium phosphate cement (CPC) and poly(lactic-co-glycolic) acid (PLGA) with different microsphere morphology (hollow vs dense). In addition to the plain CPC-PLGA composites, loading the microspheres with the growth factors platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF) was investigated. A total of four different CPC composites were applied into one-wall mandible bone defects in beagle dogs in order to evaluate them as candidates for alveolar bone regeneration. These composites consisted of CPC and hollow or dense PLGA microspheres, with or without the addition of PDGF-IGF growth factor combination (CPC-hPLGA, CPC-dPLGA, CPC-hPLGAGF , CPC-dPLGAGF ). Histological evaluation revealed significantly more bone formation in CPC-dPLGA than in CPC-hPLGA composites. The combination PDGF-IGF enhanced bone formation in CPC-hPLGA materials, but significantly more bone formation occurred when CPC-dPLGA was used, with or without the addition of growth factors. The findings demonstrated that CPC-dPLGA composite was the biologically superior material for use as an off-the-shelf material, due to its good biocompatibility, enhanced degradability and superior bone formation.
本研究旨在提供有关材料降解和随后的牙槽骨形成的信息，使用由磷酸钙水泥（CPC）和聚（乳酸-共-乙醇酸）（PLGA）组成的复合材料，具有不同的微球形态（空心与致密）。除了普通的 CPC-PLGA 复合材料外，还研究了用生长因子血小板衍生生长因子（PDGF）和胰岛素样生长因子（IGF）负载微球。总共将四种不同的 CPC 复合材料应用于比格犬的单壁下颌骨缺损中，以评估它们作为牙槽骨再生候选物的潜力。这些复合材料由 CPC 和空心或致密的 PLGA 微球组成，有或没有添加 PDGF-IGF 生长因子组合（CPC-hPLGA、CPC-dPLGA、CPC-hPLGAGF、CPC-dPLGAGF）。组织学评估显示，CPC-dPLGA 复合材料中的骨形成明显多于 CPC-hPLGA 复合材料。PDGF-IGF 组合增强了 CPC-hPLGA 材料中的骨形成，但当使用 CPC-dPLGA 时，无论是否添加生长因子，都会发生更多的骨形成。研究结果表明，CPC-dPLGA 复合材料是一种具有良好生物相容性、增强降解性和优越骨形成能力的现成材料，是生物性能更优的材料。
