Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials, and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka, 564-8680, Japan.
Rapid Commun Mass Spectrom. 2012 Aug 30;26(16):1849-58. doi: 10.1002/rcm.6301.
Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows for the simultaneous detection and imaging of several molecules in a sample. However, when using an organic matrix in the MALDI-IMS of small molecules, inhomogeneous matrix crystallization may yield poorly reproducible peaks in the mass spectra. We describe a solvent-free approach that employs a homogeneously deposited metal nanoparticle layer (or film) for small-molecule detection.
Platinum vapor deposition surface-assisted laser desorption/ionization imaging mass spectrometry (Pt vapor deposition SALDI-IMS) of small molecules was performed as a solvent-free and organic-matrix-free method. A commercially available magnetron sputtering device was used for Pt deposition. Vapor deposition of Pt produced a homogenous layer of nanoparticles over the surface of the target imaging sample.
The effectiveness of Pt vapor deposition SALDI-IMS was demonstrated for the direct detection of small analytes of inkjet ink on printed paper as well as for various other analytes (saccharides, pigments, and drugs) separated by thin-layer chromatography (TLC), without the need for extraction or concentration processes. The advantage of choosing Pt instead of Au in SALDI-IMS was also shown.
A solvent-free approach involving the direct deposition of Pt on samples (SALDI-IMS) is effective for the analysis of inkjet-printed papers and various analytes separated by TLC. This method would be useful in imaging analyses of various insulating materials such as polymers and biological materials.
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）允许在样品中同时检测和成像几种分子。然而，当在小分子的 MALDI-IMS 中使用有机基质时，不均匀的基质结晶可能导致质谱中出现重现性差的峰。我们描述了一种无溶剂方法，该方法使用均匀沉积的金属纳米粒子层（或薄膜）来检测小分子。
采用无溶剂和无有机基质的方法，进行铂蒸气沉积表面辅助激光解吸/离子化成像质谱（Pt 蒸气沉积 SALDI-IMS）检测小分子。商用磁控溅射设备用于 Pt 沉积。Pt 的蒸气沉积在目标成像样品表面上产生了均匀的纳米粒子层。
Pt 蒸气沉积 SALDI-IMS 的有效性已通过直接检测喷墨油墨在打印纸上的小分析物以及各种其他分析物（糖，颜料和药物）得到证明，而无需进行提取或浓缩过程。还显示了在 SALDI-IMS 中选择 Pt 而不是 Au 的优势。
涉及直接在样品上沉积 Pt 的无溶剂方法（SALDI-IMS）对于喷墨打印纸和通过薄层色谱（TLC）分离的各种分析物的分析是有效的。该方法将在各种绝缘材料（例如聚合物和生物材料）的成像分析中有用。
