AO Research Institute, Davos, Switzerland.
Eur Cell Mater. 2012 Jul 9;24:1-17. doi: 10.22203/ecm.v024a01.
The process of bone formation, remodelling and healing involves a coordinated action of various cell types. Advances in understanding the biology of osteoblast cells during these processes have been enabled through the use of various in vitro culture models from different origins. In an era of intensive bone tissue engineering research, these cell models are more and more often applied due to limited availability of primary human osteoblast cells. While they are a helpful tool in developing novel therapies or biomaterials; concerns arise regarding their phenotypic state and differences in relation to primary human osteoblast cells. In this review we discuss the osteoblastic development of some of the available cell models; such as primary human, rat, mouse, bovine, ovine and rabbit osteoblast cells; as well as MC3T3-E1, MG-63 and SaOs-2 cell lines, together with their advantages and disadvantages. Through this, we provide suggestions on the selection of the appropriate and most relevant osteoblast model for in vitro studies, with specific emphasis on cell-material based studies.
骨形成、重塑和修复的过程涉及各种细胞类型的协调作用。通过使用来自不同来源的各种体外培养模型，深入了解成骨细胞在这些过程中的生物学特性成为可能。在骨组织工程研究的时代，由于原发性人成骨细胞的可用性有限，这些细胞模型越来越多地被应用。虽然它们是开发新型治疗方法或生物材料的有用工具，但人们对其表型状态以及与原发性人成骨细胞的差异表示关注。在这篇综述中，我们讨论了一些可用细胞模型的成骨细胞发育，例如原代人、大鼠、小鼠、牛、绵羊和兔成骨细胞，以及 MC3T3-E1、MG-63 和 SaOs-2 细胞系，以及它们的优缺点。通过这篇综述，我们就体外研究中成骨细胞模型的选择提供了一些建议，重点是基于细胞-材料的研究。
