Bishop Ryan T, Delgado-Calle Jesus, Reagan Michaela R, Marino Silvia
Department of Tumor Microenvironment and Metastasis, H. Lee Moffitt Cancer Research Center and Institute, Tampa, FL, USA.
Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Methods Mol Biol. 2025;2885:113-141. doi: 10.1007/978-1-0716-4306-8_7.
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most used cell culture techniques, functional assays, and gene expression.
共培养分析用于研究体外细胞间的相互作用。本章描述了用于研究癌细胞与骨髓脂肪细胞、成骨细胞、破骨细胞和骨细胞之间细胞间信号串扰的二维和三维共培养系统。本章提供了最常用的细胞培养技术、功能分析和基因表达的分步指南。
