Chattrathikul Vichuda, Pinthonglor Putida, Supanchart Chayarop, Sangin Supatra
Chiang Mai University, Faculty of Dentistry, Department of Restorative Dentistry and Periodontology, Thailand.
Chiang Mai University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Thailand.
J Appl Oral Sci. 2025 May 30;33:e20240575. doi: 10.1590/1678-7757-2024-0575. eCollection 2025.
To investigate the effects of concentrated platelet-rich fibrin (C-PRF), injectable platelet-rich fibrin (i-PRF), and platelet-rich plasma (PRP) on cellular activity of human primary osteoblasts.
C-PRF, i-PRF, and PRP were prepared from five donors and pre-cultured in 5 mL of culture medium for three days. Human primary osteoblasts were seeded and cultured with 20% conditioned medium derived from the three platelet concentrates. Then, osteoblast viability was assessed at 24 h; proliferation at one, three, and five days; differentiation at seven days; mineralization at 14 days; and gene expression RUNX family transcription factor 2 (RUNX2), alkaline phosphatase, biomineralization associated (ALPL), collagen type I alpha 1 chain (COL1A1), and osteocalcin (OCN) at three and 14 days were investigated.
Osteoblasts cultured with C-PRF, i-PRF, and PRP demonstrated excellent biocompatibility. Proliferation was significantly higher in all platelet concentrates compared to the controls at one, three, and five days, with no significant differences among them, except on day one. Alkaline phosphatase and Alizarin Red S staining were significantly higher in the C-PRF and i-PRF groups compared to the PRP and control groups. However, RUNX2, ALPL, COL1A1, and OCN mRNA levels did not differ significantly among the three platelet concentrates throughout the study period.
Our study indicates that the three liquid platelet concentrates enhance human osteoblast activity. C-PRF and i-PRF promoted greater differentiation and mineralization than PRP. These findings show that all liquid platelet concentrates positively influence human osteoblast proliferation and differentiation, making them suitable for clinical applications requiring bone regeneration.
研究浓缩富血小板纤维蛋白（C-PRF）、可注射富血小板纤维蛋白（i-PRF）和富血小板血浆（PRP）对人原代成骨细胞细胞活性的影响。
从五名供体中制备C-PRF、i-PRF和PRP，并在5 mL培养基中预培养三天。接种人原代成骨细胞，并用来自三种血小板浓缩物的20%条件培养基进行培养。然后，在24小时时评估成骨细胞活力；在第1、3和5天评估增殖情况；在第7天评估分化情况；在第14天评估矿化情况；并在第3天和第14天研究RUNX家族转录因子2（RUNX2）、碱性磷酸酶、生物矿化相关（ALPL）、I型胶原α1链（COL1A1）和骨钙素（OCN）的基因表达。
用C-PRF、i-PRF和PRP培养的成骨细胞表现出优异的生物相容性。在第1、3和5天，与对照组相比，所有血小板浓缩物中的增殖均显著更高，除第1天外，它们之间无显著差异。与PRP组和对照组相比，C-PRF组和i-PRF组中的碱性磷酸酶和茜素红S染色显著更高。然而，在整个研究期间，三种血小板浓缩物之间的RUNX2、ALPL、COL1A1和OCN mRNA水平无显著差异。
我们的研究表明，这三种液体血小板浓缩物可增强人成骨细胞活性。C-PRF和i-PRF比PRP促进了更大程度的分化和矿化。这些发现表明，所有液体血小板浓缩物均对人成骨细胞增殖和分化产生积极影响，使其适用于需要骨再生的临床应用。
