Abarikwu S O, Otuechere C A, Ekor M, Monwuba K, Osobu D
Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Mowe, Ogun State, Nigeria.
Toxicol Int. 2012 May;19(2):207-14. doi: 10.4103/0971-6580.97224.
Cyclophosphamide (CYC) as an anticancer alkylating agent has been known as a male reproductive toxicant. This study was aimed to evaluate the protective effect of rutin (RUT) on CYC-induced reproductive toxicity. Sexually mature Wistar rats (weighing 199 ± 10 g with five animals in each group) were given CYC (15 mg/kg) and/or RUT (30 mg/kg) twice a week via gavage for 4 weeks. The sperm counts, sperm motility, sperm morphology, daily sperm production (DSP), testicular, and epididymal antioxidant systems: superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and testicular steroidogenic enzymes (3β-hydroxysteroid dehydrogenase and 17β-HSD and spermatogenesis marker enzymes (lactate dehydrogenase (LDH), sorbitol dehydrogenase (SDH), alkaline phosphatase (ALP), acid phosphatase (ACP) in the testes, epididymis and seminal vesicles were investigated at the end of the fourth week. By the end of the fourth week, RUT prevented lower sperm counts, sperm motility, DSP, and higher abnormal sperm numbers induced by CYC. In testes, RUT decreased SOD, LDH, and SDH and increased CAT, 3β-HSD, 17β-HSD, ALP, and ACP induced by CYC. In epididymis, RUT increased SOD, CAT, GSH, GSH-Px, GR, GST SDH, ALP and ACP and decreased MDA and LDH induced by CYC. In seminal vesicles, marker enzymes were unchanged in rats given CYC alone or in combination with RUT. It appears that RUT ameliorates CYC reproductive toxicity at the investigated dose.
环磷酰胺（CYC）作为一种抗癌烷化剂，一直被认为是一种雄性生殖毒物。本研究旨在评估芦丁（RUT）对CYC诱导的生殖毒性的保护作用。性成熟的Wistar大鼠（体重199±10克，每组五只动物）每周经口灌胃给予CYC（15毫克/千克）和/或RUT（30毫克/千克）两次，持续4周。在第四周结束时，对精子数量、精子活力、精子形态、每日精子生成量（DSP）、睾丸和附睾抗氧化系统：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶（GST）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）以及睾丸类固醇生成酶（3β-羟基类固醇脱氢酶和17β-羟类固醇脱氢酶）和生精标记酶（乳酸脱氢酶（LDH）、山梨醇脱氢酶（SDH）、碱性磷酸酶（ALP）、酸性磷酸酶（ACP））在睾丸、附睾和精囊中的情况进行了研究。到第四周结束时，RUT预防了CYC诱导的精子数量减少、精子活力下降、DSP降低以及异常精子数量增加。在睾丸中，RUT降低了CYC诱导的SOD、LDH和SDH，并增加了CAT、3β-HSD、17β-HSD、ALP和ACP。在附睾中，RUT增加了CYC诱导的SOD、CAT、GSH、GSH-Px、GR、GST、SDH、ALP和ACP，并降低了MDA和LDH。在精囊中，单独给予CYC或与RUT联合给予的大鼠中，标记酶没有变化。在所研究的剂量下，RUT似乎改善了CYC的生殖毒性。
