Abarikwu Sunny O, Farombi Ebenezer O
Department of Biochemistry, College of Natural and Applied Sciences, University of Port Harcourt, Choba, Nigeria
Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Toxicol Ind Health. 2016 Jul;32(7):1278-85. doi: 10.1177/0748233714555389. Epub 2014 Nov 26.
The protective effect of quercetin (QT) on atrazine (ATZ)-induced testicular damage in rats was investigated. Sexually mature male Wistar rats (weighing 220-250 g) divided into four groups with six animals in each group were given ATZ (120 mg kg(-1); 1/16 of the median lethal dose for an oral dose) and/or QT (10 mg kg(-1)) daily via gavage for 16 days. By the end of day 16, rats given ATZ alone had significantly lower sperm counts, daily spermatozoa production, and sperm motility and significantly higher abnormal sperm numbers than the untreated control rats. The rats given ATZ alone also had significantly decreased 3β-hydroxtsteroid dehydrogenase (HSD) and 17β-HSD activities than the control rats. Lactate dehydrogenase activity and malondialdehyde levels were significantly increased, whereas superoxide dismutase activity decreased but glutathione levels remain unaffected after ATZ exposure. These changes were reversed toward control values in the QT + ATZ-treated animals, though the sperm motility was 28% below the control levels but was still higher than in the ATZ-treated rats. The results indicate that QT might improve testicular function of rats exposed to ATZ, but its protective effect on sperm motility might be partial.
研究了槲皮素（QT）对大鼠阿特拉津（ATZ）诱导的睾丸损伤的保护作用。将性成熟雄性Wistar大鼠（体重220 - 250 g）分为四组，每组6只动物，每天通过灌胃给予ATZ（120 mg kg⁻¹；口服剂量的半数致死剂量的1/16）和/或QT（10 mg kg⁻¹），持续16天。在第16天结束时，单独给予ATZ的大鼠的精子计数、每日精子生成量和精子活力显著低于未处理的对照大鼠，异常精子数量显著高于对照大鼠。单独给予ATZ的大鼠的3β - 羟基类固醇脱氢酶（HSD）和17β - HSD活性也比对照大鼠显著降低。ATZ暴露后，乳酸脱氢酶活性和丙二醛水平显著升高，而超氧化物歧化酶活性降低，但谷胱甘肽水平未受影响。在QT + ATZ处理的动物中，这些变化向对照值逆转，尽管精子活力比对照水平低28%，但仍高于ATZ处理的大鼠。结果表明，QT可能改善暴露于ATZ的大鼠的睾丸功能，但其对精子活力的保护作用可能是部分的。
