东方精神病患者红细胞中氟哌啶醇还原酶活性及血浆中还原型氟哌啶醇/氟哌啶醇比率的测定。

Measurement of haloperidol reductase activity in red blood cells and reduced haloperidol/haloperidol ratios in plasma in oriental psychiatric patients.

Shibasaki M, Someya T, Kato T, Noguchi T, Ishida N, Takahashi S

Department of Psychiatry, Shiga University of Medical Science, Otsu, Japan.

Prog Neuropsychopharmacol Biol Psychiatry. 1990;14(6):941-7. doi: 10.1016/0278-5846(90)90079-v.

Abstract

The authors established a method for measuring haloperidol (HAL) reductase activity in human red blood cells. 2. Characteristics of the HAL reductase in red blood cells were examined. This enzyme reaction was NADPH dependent, and the optimum pH was at 8.2-8.9. Vmax and Km were calculated as 25-150 pmol/hr/10(6) RBC and 160-2600 microM respectively. 3. HAL reductase activities in red blood cells from 14 patients treated with HAL were in a range of 9.7-20.8 pmol/hr/10(6) RBC. So far we did not find any significant correlation between HAL reductase activities and reduced HAL/HAL ratios in plasma.

摘要

作者建立了一种测量人红细胞中氟哌啶醇（HAL）还原酶活性的方法。2. 对红细胞中HAL还原酶的特性进行了研究。该酶反应依赖于NADPH，最适pH值为8.2 - 8.9。Vmax和Km分别计算为25 - 150 pmol/小时/10(6)个红细胞和160 - 2600微摩尔。3. 14例接受HAL治疗患者的红细胞中HAL还原酶活性在9.7 - 20.8 pmol/小时/10(6)个红细胞范围内。到目前为止，我们尚未发现HAL还原酶活性与血浆中HAL还原型/HAL比值之间存在任何显著相关性。