Jiang Xiao-dong, Qiao Yun, Dai Peng, Chen Qin, Wu Jin, Song Da-an, Li Shi-qiu
Department of Radiology, Lianyungang First People's Hospital, Jiangsu, Lianyungang 222002, China.
J Biomed Biotechnol. 2012;2012:301931. doi: 10.1155/2012/301931. Epub 2012 Jun 18.
We observed the effects of endostar on the radiosensitivity of pulmonary adenocarcinoma A549 cells and found that endostar inhibited A549 cell growth under normoxia and hypoxia in time and dose-dependent manners; the D(0) and D(q) values in control and endostar groups were (1.36 and 1.30) versus (1.019 and 1.015) under normoxia and (1.693 and 1.39) versus (2.453 and 1.026) under hypoxia, respectively; SER was 1.04 under normoxia and 1.22 under hypoxia in endostar group; under normoxia, the apoptosis rates in control, radiotherapy, endostar and combination groups were 15.9 ± 0.57%, 42.7 ± 0.37%, 19.9 ± 0.48%, and 41.5 ± 0.38%, respectively, with no significant difference between combination and radiotherapy groups; there was significant difference in G(2)/M phase cells between combination and radiotherapy groups (P = 0.028); under hypoxia, the apoptosis rates in the four groups were 16.7 ± 0.67%, 30.1 ± 0.95%, 26.7 ± 0.62%, and 36.3 ± 0.71%, respectively, with significant difference between combination and radiotherapy groups; G(2)/M phase cells were higher in combination group than radiotherapy group (P = 0.000); G(2)/M phase cells were higher in hypoxic combination group than in normoxic combination group (P = 0.003). Based on these results, we conclude that under hypoxia, endostar can enhance the radiosensitivity of A549 cells through G(2)/M arrest.
我们观察了恩度对肺腺癌A549细胞放射敏感性的影响，发现恩度在常氧和缺氧条件下均能以时间和剂量依赖性方式抑制A549细胞生长；常氧条件下对照组和恩度组的D(0)和D(q)值分别为(1.36和1.30)与(1.019和1.015)，缺氧条件下分别为(1.693和1.39)与(2.453和1.026)；恩度组常氧时的增敏比为1.04，缺氧时为1.22；常氧条件下，对照组、放疗组、恩度组及联合组的凋亡率分别为15.9±0.57%、42.7±0.37%、19.9±0.48%和41.5±0.38%，联合组与放疗组之间无显著差异；联合组与放疗组在G(2)/M期细胞方面存在显著差异(P = 0.028)；缺氧条件下，四组的凋亡率分别为16.7±0.67%、30.1±0.95%、26.7±0.62%和36.3±0.71%，联合组与放疗组之间存在显著差异；联合组的G(2)/M期细胞高于放疗组(P = 0.000)；缺氧联合组的G(2)/M期细胞高于常氧联合组(P = 0.003)。基于这些结果，我们得出结论，在缺氧条件下，恩度可通过使G(2)/M期阻滞来增强A549细胞的放射敏感性。
