Department of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea.
Korean J Radiol. 2012 Jul-Aug;13(4):450-7. doi: 10.3348/kjr.2012.13.4.450. Epub 2012 Jun 18.
To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and (18)F-fluorodeoxyglucose-positron emission tomography ((18)F-FDG-PET) imaging of collagen-induced arthritis (CIA).
We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq (18)F-FDG in 200 µL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice.
The mean standardized uptake values of (18)F-FDG for knees and ankles were 1.68 ± 0.76 and 0.79 ± 0.71, respectively, for CIA mice; and 0.57 ± 0.17 and 0.54 ± 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm(2) for knees and ankles were 2.32 ± 1.54 × 10(5) and 2.75 ± 1.51 × 10(5), respectively, for CIA mice, and 1.22 ± 0.27 × 10(5) and 0.88 ± 0.24 × 10(5), respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and (18)F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice.
NIRF imaging using HGC-Cy5.5 was moderately correlated with (18)F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.
评估近红外荧光（NIRF）成像使用菁染料 5.5 与疏水性修饰的乙二醇壳聚糖纳米粒子（HGC-Cy5.5）的潜在关联，并评估其与胶原诱导关节炎（CIA）的（18）F-氟脱氧葡萄糖正电子发射断层扫描（（18）F-FDG-PET）成像的关联。
我们使用了 10 只 CIA 模型鼠和 3 只正常鼠。在两次注射胶原后 9 天，在静脉注射 9.3 MBq（18）F-FDG 于 200μL PBS 后 40 分钟进行 microPET 成像。一天后，在静脉注射 HGC-cy5.5（5mg/kg）后 2 小时进行 NIRF 成像。我们评估了 CIA 模型鼠膝关节和踝关节中这两种模式之间的相关性。
膝关节和踝关节的（18）F-FDG 标准化摄取值（SUV）分别为 CIA 模型鼠的 1.68 ± 0.76 和 0.79 ± 0.71，正常鼠的 0.57 ± 0.17 和 0.54 ± 0.20。从 NIRF 图像来看，膝关节和踝关节每 30mm2 的总光子计数分别为 CIA 模型鼠的 2.32 ± 1.54×105 和 2.75 ± 1.51×105，正常鼠的 1.22 ± 0.27×105 和 0.88 ± 0.24×105。这两种模式在膝关节（r=0.604，p=0.005）和踝关节（r=0.464，p=0.039）均显示出中度相关性。此外，HGC-Cy5.5（p=0.002）和（18）F-FDG-PET（p=0.005）成像在 CIA 模型鼠和正常鼠之间也显示出统计学显著差异。
HGC-Cy5.5 近红外荧光成像与 CIA 模型中的（18）F-FDG-PET 成像具有中度相关性。因此，HGC-Cy5.5 成像可用于类风湿关节炎的早期检测。
