Cohen Gadi, Natsheh Hiba, Sunny Youhan, Bawiec Christopher R, Touitou Elka, Lerman Melissa A, Lazarovici Philip, Lewin Peter A
School of Pharmacy Institute for Drug Research, Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
School of Biomedical Engineering, Sciences and Heath Systems, Drexel University, Philadelphia, Pennsylvania, USA.
Ultrasound Med Biol. 2015 Sep;41(9):2449-57. doi: 10.1016/j.ultrasmedbio.2015.04.009. Epub 2015 May 21.
The purpose of this work was to investigate whether low-frequency, low-intensity (20 kHz, <100 mW/cm(2), spatial-peak, temporal-peak intensity) ultrasound, delivered with a lightweight (<100 g), tether-free, fully wearable, battery-powered applicator, is capable of reducing inflammation in a mouse model of rheumatoid arthritis. The therapeutic, acute, anti-inflammatory effect was estimated from the relative swelling induced in mice hindlimb paws. In an independent, indirect approach, the inflammation was bio-imaged by measuring glycolytic activity with near-infrared labeled 2-deoxyglucose. The outcome of the experiments indicated that the combination of ultrasound exposure and topical application of 0.1% (w/w) betamethasone gel resulted in statistically significantly (p < 0.05) enhanced anti-inflammatory activity in comparison with drug or ultrasound treatment alone. The present study underscores the potential benefits of low-frequency, low-intensity ultrasound-assisted drug delivery. However, the proof of concept presented indicates the need for additional experiments to systematically evaluate and optimize the potential of, and the conditions for, tolerable low-frequency, low-intensity ultrasound-promoted non-invasive drug delivery.
本研究的目的是探讨使用一种重量轻（<100克）、无束缚、完全可穿戴、电池供电的装置，以低频、低强度（20千赫，<100毫瓦/平方厘米，空间峰值-时间峰值强度）发射超声波，是否能够减轻类风湿性关节炎小鼠模型中的炎症。通过测量小鼠后肢爪子的相对肿胀来评估治疗性、急性抗炎效果。采用一种独立的间接方法，通过用近红外标记的2-脱氧葡萄糖测量糖酵解活性对炎症进行生物成像。实验结果表明，与单独使用药物或超声波治疗相比，超声波照射与局部应用0.1%（w/w）倍他米松凝胶相结合，在统计学上具有显著增强的抗炎活性（p<0.05）。本研究强调了低频、低强度超声辅助药物递送的潜在益处。然而，目前所展示的概念验证表明，需要进行更多实验，以系统地评估和优化可耐受的低频、低强度超声促进的非侵入性药物递送的潜力和条件。
