• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过化学发光探针法（Pholasin法）评估钛、Ti6Al4V和不锈钢在免疫细胞系统中诱导产生自由基的情况。

Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay.

作者信息

P Cachinho Sandra C, Pu Fanrong

机构信息

Clinical Engineering, University of Liverpool, Liverpool L69 3GA, UK.

出版信息

Int J Biomater. 2012;2012:380845. doi: 10.1155/2012/380845. Epub 2012 Jun 19.

DOI:10.1155/2012/380845
PMID:22778739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3388343/
Abstract

The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20 μm in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent.

摘要

使用化学发光分析法研究了在不同金属材料存在下人体血细胞的氧化爆发。使用了尺寸小于20μm的颗粒形式的商业纯钛（Ti）、钛合金（Ti6Al4V）和不锈钢316L（SS）。还评估了颗粒材料调理作用对血细胞呼吸爆发产物上调的影响。同时注射刺激剂fMLP+PMA后实现了最大的化学发光响应。此外，与Ti6Al4V相比，Ti和SS诱导了更大的炎症反应，因为在调理作用处理后，这两种材料的呼吸爆发都更高。这些结果表明，体外化学发光响应和呼吸爆发测量结果证明取决于成分和处理方式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/14cc1562ab29/IJBM2012-380845.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/aba69689e2e6/IJBM2012-380845.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/6a049a38049f/IJBM2012-380845.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/527df107cf33/IJBM2012-380845.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/f4ffc755f538/IJBM2012-380845.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/14cc1562ab29/IJBM2012-380845.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/aba69689e2e6/IJBM2012-380845.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/6a049a38049f/IJBM2012-380845.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/527df107cf33/IJBM2012-380845.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/f4ffc755f538/IJBM2012-380845.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82dd/3388343/14cc1562ab29/IJBM2012-380845.005.jpg

相似文献

1
Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay.通过化学发光探针法（Pholasin法）评估钛、Ti6Al4V和不锈钢在免疫细胞系统中诱导产生自由基的情况。
Int J Biomater. 2012;2012:380845. doi: 10.1155/2012/380845. Epub 2012 Jun 19.
2
Retracted: Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay.撤回：通过化学发光探针Pholasin测定法评估钛、Ti6Al4V和不锈钢在免疫细胞系统中诱导产生自由基的情况。
Int J Biomater. 2013;2013:148185. doi: 10.1155/2013/148185. Epub 2013 Sep 9.
3
Is galvanic corrosion between titanium alloy and stainless steel spinal implants a clinical concern?钛合金与不锈钢脊柱植入物之间的电偶腐蚀是一个临床问题吗？
Spine J. 2004 Jul-Aug;4(4):379-87. doi: 10.1016/j.spinee.2003.12.004.
4
Evaluation of systemic metal diffusion after spinal pedicular fixation with titanium alloy and stainless steel system: a 36-month experimental study in sheep.钛合金和不锈钢系统脊柱椎弓根固定术后全身金属扩散的评估：一项在绵羊身上进行的36个月实验研究。
Int J Artif Organs. 2001 Jan;24(1):41-9.
5
Comparison between 4.0-mm stainless steel and 4.75-mm titanium alloy single-rod spinal instrumentation for anterior thoracoscopic scoliosis surgery.用于前路胸腔镜脊柱侧弯手术的4.0毫米不锈钢单棒与4.75毫米钛合金单棒脊柱内固定器械的比较。
Spine (Phila Pa 1976). 2008 Sep 15;33(20):2173-8. doi: 10.1097/BRS.0b013e31817f9415.
6
Commercially pure titanium (cp-Ti) versus titanium alloy (Ti6Al4V) materials as bone anchored implants - Is one truly better than the other?作为骨锚定植入物的商业纯钛（cp-Ti）与钛合金（Ti6Al4V）材料——一种真的比另一种更好吗？
Mater Sci Eng C Mater Biol Appl. 2016 May;62:960-6. doi: 10.1016/j.msec.2016.01.032. Epub 2016 Jan 16.
7
Elastic stable intramedullary nailing (ESIN) in paediatric femur and tibia shaft fractures: Comparison between titanium and stainless steel nails.小儿股骨干和胫骨干骨折的弹性稳定髓内钉固定术（ESIN）：钛钉与不锈钢钉的比较
Injury. 2018 Nov;49 Suppl 3:S8-S11. doi: 10.1016/j.injury.2018.09.049.
8
Titanium-alloy enhances bone-pedicle screw fixation: mechanical and histomorphometrical results of titanium-alloy versus stainless steel.钛合金增强椎弓根螺钉固定效果：钛合金与不锈钢的力学及组织形态计量学结果
Eur Spine J. 2000 Apr;9(2):97-103. doi: 10.1007/s005860050218.
9
Comparative in vitro biocompatibility of nickel-titanium, pure nickel, pure titanium, and stainless steel: genotoxicity and atomic absorption evaluation.镍钛合金、纯镍、纯钛和不锈钢的体外生物相容性比较：遗传毒性和原子吸收评估。
Biomed Mater Eng. 1999;9(1):1-12.
10
In vitro corrosion behavior of bioceramic, metallic, and bioceramic-metallic coated stainless steel dental implants.生物陶瓷、金属及生物陶瓷-金属涂层不锈钢牙科植入物的体外腐蚀行为
Dent Mater. 2003 May;19(3):188-98. doi: 10.1016/s0109-5641(02)00029-5.

引用本文的文献

1
Innate Immunity and Biomaterials at the Nexus: Friends or Foes.先天免疫与生物材料的关系：是友还是敌。
Biomed Res Int. 2015;2015:342304. doi: 10.1155/2015/342304. Epub 2015 Jul 12.
2
Retracted: Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay.撤回：通过化学发光探针Pholasin测定法评估钛、Ti6Al4V和不锈钢在免疫细胞系统中诱导产生自由基的情况。
Int J Biomater. 2013;2013:148185. doi: 10.1155/2013/148185. Epub 2013 Sep 9.

本文引用的文献

1
UHMWPE oxidation increases granulocytes activation: a role in tissue response after prosthesis implant.
Biomaterials. 2003 Aug;24(17):2895-900. doi: 10.1016/s0142-9612(03)00128-5.
2
Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling.活性氧与细胞信号传导：巨噬细胞信号传导中的呼吸爆发
Am J Respir Crit Care Med. 2002 Dec 15;166(12 Pt 2):S4-8. doi: 10.1164/rccm.2206007.
3
Macrophage signaling and respiratory burst.巨噬细胞信号传导与呼吸爆发。
Immunol Res. 2002;26(1-3):95-105. doi: 10.1385/IR:26:1-3:095.
4
The influence of plasma proteins and platelets on oxygen radical production and F-actin distribution in neutrophils adhering to polymer surfaces.血浆蛋白和血小板对黏附于聚合物表面的中性粒细胞中氧自由基产生及F-肌动蛋白分布的影响。
Biomaterials. 2002 Apr;23(8):1785-95. doi: 10.1016/s0142-9612(01)00305-2.
5
Respiratory burst response of peritoneal leukocytes adhering to titanium and stainless steel.腹膜白细胞对钛和不锈钢的呼吸爆发反应。
J Biomed Mater Res. 2001 Nov;57(2):238-47. doi: 10.1002/1097-4636(200111)57:2<238::aid-jbm1164>3.0.co;2-2.
6
Interactions between human whole blood and modified TiO2-surfaces: influence of surface topography and oxide thickness on leukocyte adhesion and activation.人全血与改性二氧化钛表面之间的相互作用：表面形貌和氧化物厚度对白细胞黏附及活化的影响。
Biomaterials. 2001 Jul;22(14):1987-96. doi: 10.1016/s0142-9612(00)00382-3.
7
Immunoglobulin enhances the bioactive-glass-induced chemiluminescence response of human polymorphonuclear leukocytes.免疫球蛋白增强生物活性玻璃诱导的人多形核白细胞化学发光反应。
J Biomed Mater Res. 2001 Jun 15;55(4):613-7. doi: 10.1002/1097-4636(20010615)55:4<613::aid-jbm1055>3.0.co;2-h.
8
Free radical-mediated transgene inactivation of macrophages by endotoxin.内毒素介导的巨噬细胞自由基介导的转基因失活
Am J Physiol Lung Cell Mol Physiol. 2000 Nov;279(5):L878-83. doi: 10.1152/ajplung.2000.279.5.L878.
9
Neutrophil activation by plasma opsonized polymeric microspheres: inhibitory effect of pluronic F127.血浆调理素化聚合物微球对中性粒细胞的激活作用：普朗尼克F127的抑制作用
Biomaterials. 2000 Jul;21(14):1483-91. doi: 10.1016/s0142-9612(00)00034-x.
10
Secretion of IL-1 and H2O2 by human mononuclear cells in vitro.人单核细胞在体外分泌白细胞介素-1和过氧化氢。
Biomaterials. 2000 May;21(10):1047-55. doi: 10.1016/s0142-9612(99)00279-3.