• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从海洋海绵 Halichondria okadai 中分离得到的 18 kDa 凝集素的细胞毒性和糖结合特性。

Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai.

机构信息

Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.

出版信息

Toxins (Basel). 2012 May;4(5):323-38. doi: 10.3390/toxins4050323. Epub 2012 Apr 30.

DOI:10.3390/toxins4050323
PMID:22778903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3386633/
Abstract

A divalent cation-independent lectin-HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc), fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4-12 and temperatures lower than 60 °C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for N-linked complex-type and sphingolipid-type oligosaccharides with N-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.

摘要

一种二价阳离子非依赖性凝集素-HOL-18，具有细胞毒性活性，可针对白血病细胞，从软海绵 Halichondria okadai 中纯化出来。HOL-18 是一种 72 kDa 的四聚体凝集素，由四个非共价结合的 18 kDa 亚基组成。该凝集素的血凝活性受到壳三糖（GlcNAcβ1-4GlcNAcβ1-4GlcNAc）、胎球蛋白和牛颌下腺粘蛋白的强烈抑制。该凝集素在 pH4-12 和低于 60°C 的温度下稳定。与 16 种吡啶氨化寡糖的前沿亲和层析表明，该凝集素对 N-连接的复合型和神经酰胺型寡糖具有亲和力，这些寡糖在非还原末端具有 N-乙酰化己糖胺和神经氨酸。该凝集素以剂量和碳水化合物依赖的方式杀死 Jurkat 白血病 T 细胞和 K562 红细胞白血病细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/c66631631f9e/toxins-04-00323-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/95c20dcede88/toxins-04-00323-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/8c9dd4f75a95/toxins-04-00323-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/6dc19f51f64b/toxins-04-00323-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/82334ba3d0f1/toxins-04-00323-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/156020f1c6ab/toxins-04-00323-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/93780bc386af/toxins-04-00323-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/c66631631f9e/toxins-04-00323-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/95c20dcede88/toxins-04-00323-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/8c9dd4f75a95/toxins-04-00323-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/6dc19f51f64b/toxins-04-00323-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/82334ba3d0f1/toxins-04-00323-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/156020f1c6ab/toxins-04-00323-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/93780bc386af/toxins-04-00323-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4c2/3386633/c66631631f9e/toxins-04-00323-g007.jpg

相似文献

1
Cytotoxicity and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halichondria okadai.从海洋海绵 Halichondria okadai 中分离得到的 18 kDa 凝集素的细胞毒性和糖结合特性。
Toxins (Basel). 2012 May;4(5):323-38. doi: 10.3390/toxins4050323. Epub 2012 Apr 30.
2
Two lectins from the marine sponge Halichondria okadai. An N-acetyl-sugar-specific lectin (HOL-I) and an N-acetyllactosamine-specific lectin (HOL-II).来自海洋海绵冈村氏软海绵的两种凝集素。一种N-乙酰糖特异性凝集素（HOL-I）和一种N-乙酰乳糖胺特异性凝集素（HOL-II）。
J Biol Chem. 1994 Jan 14;269(2):1375-9.
3
Histochemical localization of N-acetylhexosamine-binding lectin HOL-18 in Halichondria okadai (Japanese black sponge), and its antimicrobial and cytotoxic anticancer effects.在日本黑海绵（Halichondria okadai）中 N-乙酰己糖胺结合凝集素 HOL-18 的组织化学定位及其抗菌和细胞毒性抗癌作用。
Int J Biol Macromol. 2019 Mar 1;124:819-827. doi: 10.1016/j.ijbiomac.2018.11.222. Epub 2018 Nov 26.
4
Isolation, purification, characterization and glycan-binding profile of a d-galactoside specific lectin from the marine sponge, Halichondria okadai.从海洋海绵冲绳软海绵中分离、纯化、表征及d-半乳糖苷特异性凝集素的聚糖结合谱
Comp Biochem Physiol B Biochem Mol Biol. 2008 Aug;150(4):349-57. doi: 10.1016/j.cbpb.2008.04.004. Epub 2008 Apr 16.
5
Lectin, a possible basis for symbiosis between bacteria and sponges.凝集素，细菌与海绵共生的一种可能基础。
J Bacteriol. 1981 Jan;145(1):548-58. doi: 10.1128/jb.145.1.548-558.1981.
6
Glycomics of a novel type-2 N-acetyllactosamine-specific lectin purified from the feather star, Oxycomanthus japonicus (Pelmatozoa: Crinoidea).从日本羽星（海百合纲：海百合目）中纯化出的新型 2 型 N-乙酰乳糖胺特异性凝集素的糖组学研究。
Comp Biochem Physiol B Biochem Mol Biol. 2011 Apr;158(4):266-73. doi: 10.1016/j.cbpb.2010.12.004. Epub 2010 Dec 19.
7
Cytotoxicity and glycan-binding profile of a D-galactose-binding lectin from the eggs of a Japanese sea hare (Aplysia kurodai).日本海兔（Aplysia kurodai）卵中 D-半乳糖结合凝集素的细胞毒性和聚糖结合特性。
Protein J. 2011 Oct;30(7):509-19. doi: 10.1007/s10930-011-9356-7.
8
Isolation, Purification and Characterisation of a D-galactose and N-acetyl-D-galactosamine Specific Lectin from Marine Sponge Fasciospongia cavernosa.从海洋海绵Fasciospongia cavernosa中分离、纯化和鉴定一种D-半乳糖和N-乙酰-D-半乳糖胺特异性凝集素。
Protein Pept Lett. 2018;25(9):871-877. doi: 10.2174/0929866525666180905111452.
9
A potent mitogenic lectin from the mycelia of a phytopathogenic fungus, Rhizoctonia bataticola, with complex sugar specificity and cytotoxic effect on human ovarian cancer cells.一种来自植物病原菌茄病镰刀菌菌丝体的具有复杂糖特异性的有丝分裂原凝集素，对人卵巢癌细胞具有细胞毒性作用。
Glycoconj J. 2010 Apr;27(3):375-86. doi: 10.1007/s10719-010-9285-2. Epub 2010 Mar 22.
10
Purification, characterization and biological effect of lectin from the marine sponge Stylissa flexibilis (Lévi, 1961).来自柔韧扁海绵（Lévi，1961年）的凝集素的纯化、表征及生物学效应
Comp Biochem Physiol B Biochem Mol Biol. 2018 Feb;216:32-38. doi: 10.1016/j.cbpb.2017.11.008. Epub 2017 Nov 22.

引用本文的文献

1
Unveiling Palmitoyl Thymidine Derivatives as Antimicrobial/Antiviral Inhibitors: Synthesis, Molecular Docking, Dynamic Simulations, ADMET, and Assessment of Protein-Ligand Interactions.揭示棕榈酰胸苷衍生物作为抗菌/抗病毒抑制剂：合成、分子对接、动力学模拟、ADMET 及蛋白质-配体相互作用评估
Pharmaceuticals (Basel). 2025 May 27;18(6):806. doi: 10.3390/ph18060806.
2
Synthesis, Antimicrobial Activity, DFT, Molecular Docking, and Dynamic Simulations of Trityl Mannopyranoside Derivatives for Potential Antibacterial Agents.用于潜在抗菌剂的三苯甲基甘露吡喃糖苷衍生物的合成、抗菌活性、密度泛函理论、分子对接及动力学模拟
Med Chem. 2025;21(5):403-424. doi: 10.2174/0115734064339243241027024304.
3

本文引用的文献

1
Diversified carbohydrate-binding lectins from marine resources.来自海洋资源的多样化碳水化合物结合凝集素。
J Amino Acids. 2011;2011:838914. doi: 10.4061/2011/838914. Epub 2011 Nov 15.
2
Interactions of halichondrin B and eribulin with tubulin.卤泛群 B 和埃博霉素与微管蛋白的相互作用。
J Chem Inf Model. 2011 Jun 27;51(6):1393-404. doi: 10.1021/ci200077t. Epub 2011 May 13.
3
Synthesis, inhibitory effects on nitric oxide and structure-activity relationships of a glycosphingolipid from the marine sponge Aplysinella rhax and its analogues.
Marine Lectins and Lectin-like Proteins as Promising Molecules Targeting Aberrant Glycosylation Signatures in Human Brain Tumors.
海洋凝集素和凝集素样蛋白作为靶向人类脑肿瘤异常糖基化特征的有前景的分子。
Mar Drugs. 2024 Nov 22;22(12):527. doi: 10.3390/md22120527.
4
In vitro antimicrobial, anticancer evaluation, and in silico studies of mannopyranoside analogs against bacterial and fungal proteins: Acylation leads to improved antimicrobial activity.吡喃甘露糖苷类似物对细菌和真菌蛋白质的体外抗菌、抗癌评估及计算机模拟研究：酰化作用可提高抗菌活性。
Saudi Pharm J. 2024 Jun;32(6):102093. doi: 10.1016/j.jsps.2024.102093. Epub 2024 Apr 30.
5
Marine-Derived Compounds as Potential Inhibitors of Hsp90 for Anticancer and Antimicrobial Drug Development: A Comprehensive In Silico Study.海洋来源化合物作为 Hsp90 的潜在抑制剂在抗癌和抗菌药物开发中的应用：全面的计算机研究。
Molecules. 2023 Dec 13;28(24):8074. doi: 10.3390/molecules28248074.
6
An Update of Lectins from Marine Organisms: Characterization, Extraction Methodology, and Potential Biofunctional Applications.海洋生物凝集素：特性、提取方法及潜在生物功能应用的最新研究进展。
Mar Drugs. 2022 Jun 29;20(7):430. doi: 10.3390/md20070430.
7
Marine Demospongiae: A Challenging Treasure of Bioactive Compounds.海洋寻常海绵：生物活性化合物的挑战性宝藏。
Mar Drugs. 2022 Mar 31;20(4):244. doi: 10.3390/md20040244.
8
Diverse Localization Patterns of an R-Type Lectin in Marine Annelids.海洋环节动物中 R 型凝集素的多样化定位模式。
Molecules. 2021 Aug 7;26(16):4799. doi: 10.3390/molecules26164799.
9
Antitumor Potential of Marine and Freshwater Lectins.海洋和淡水凝集素的抗肿瘤潜力。
Mar Drugs. 2019 Dec 21;18(1):11. doi: 10.3390/md18010011.
10
Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.来自九齿扇虾（拖鞋龙虾）的一种新型巨大凝集素的内化诱导哺乳动物癌细胞凋亡。
Glycoconj J. 2017 Feb;34(1):85-94. doi: 10.1007/s10719-016-9731-x. Epub 2016 Sep 22.
海洋海绵 Aplysinella rhax 糖脂及其类似物的合成、对一氧化氮的抑制作用及构效关系研究。
Molecules. 2011 Jan 17;16(1):637-51. doi: 10.3390/molecules16010637.
4
Globotriaosylceramide-expressing Burkitt's lymphoma cells are committed to early apoptotic status by rhamnose-binding lectin from catfish eggs.表达球三糖神经酰胺的伯基特淋巴瘤细胞会被来自鲶鱼卵的鼠李糖结合凝集素诱导进入早期凋亡状态。
Biol Pharm Bull. 2009 Mar;32(3):345-53. doi: 10.1248/bpb.32.345.
5
Analysis of N-glycans in embryonated chicken egg chorioallantoic and amniotic cells responsible for binding and adaptation of human and avian influenza viruses.对鸡胚绒毛尿囊膜和羊膜细胞中负责人类和禽流感病毒结合与适应的N-聚糖的分析。
Glycoconj J. 2009 May;26(4):433-43. doi: 10.1007/s10719-008-9193-x. Epub 2008 Oct 14.
6
High affinity interaction between a bivalve C-type lectin and a biantennary complex-type N-glycan revealed by crystallography and microcalorimetry.晶体学和微量热法揭示双壳贝类C型凝集素与双天线复合型N-聚糖之间的高亲和力相互作用。
J Biol Chem. 2008 Oct 31;283(44):30112-20. doi: 10.1074/jbc.M804353200. Epub 2008 Aug 7.
7
Isolation, purification, characterization and glycan-binding profile of a d-galactoside specific lectin from the marine sponge, Halichondria okadai.从海洋海绵冲绳软海绵中分离、纯化、表征及d-半乳糖苷特异性凝集素的聚糖结合谱
Comp Biochem Physiol B Biochem Mol Biol. 2008 Aug;150(4):349-57. doi: 10.1016/j.cbpb.2008.04.004. Epub 2008 Apr 16.
8
ACL-I, a lectin from the marine sponge Axinella corrugata: isolation, characterization and chemotactic activity.ACL-I，一种来自海洋海绵皱波角海绵的凝集素：分离、表征及趋化活性。
Comp Biochem Physiol C Toxicol Pharmacol. 2008 Jul;148(1):23-30. doi: 10.1016/j.cbpc.2008.03.003. Epub 2008 Mar 12.
9
Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus.使用在线流动聚糖释放装置对粘蛋白型聚糖进行快速灵敏分析。
Anal Biochem. 2007 Dec 1;371(1):52-61. doi: 10.1016/j.ab.2007.06.013. Epub 2007 Jun 15.
10
First evidence of chitin as a component of the skeletal fibers of marine sponges. Part I. Verongidae (demospongia: Porifera).几丁质作为海洋海绵骨骼纤维成分的首个证据。第一部分。Verongidae（寻常海绵纲：多孔动物门）。
J Exp Zool B Mol Dev Evol. 2007 Jul 15;308(4):347-56. doi: 10.1002/jez.b.21156.