Department of Laboratory Medicine, Ulsan University Hospital, University of Ulsan College of Medicine, Korea.
Ann Lab Med. 2012 Jul;32(4):298-303. doi: 10.3343/alm.2012.32.4.298. Epub 2012 Jun 20.
A slowly growing, non-chromogenic mycobacterial strain was isolated from sputum and bronchial lavage fluid samples of a patient presenting with productive cough, blood-tinged sputum, low-grade fever, and weakness. A positive acid-fast bacilli sputum smear result prompted the initiation of an anti-tuberculosis regimen. Multiplex real-time PCR showed a negative result for Mycobacterium tuberculosis complex and a positive result for nontuberculous mycobacteria. The DNA chip test confirmed this organism as a member of the genus Mycobacterium, but could not specify the species. Interestingly, the mycolic acid patterns obtained by HPLC nearly overlapped with those of M. simulans. The sequences of the Mycobacterium 16S rRNA gene and 16S-23S internal transcribed spacer region were unique and were found to have 100% similarity with those of M. riyadhense. After a review of the literature, we report this case as the first Korean case of M. riyadhense lung infection.
一株生长缓慢、非显色分枝杆菌菌株从一位出现咳嗽有痰、痰中带血、低热和乏力的患者的痰和支气管灌洗液样本中分离出来。阳性抗酸杆菌痰涂片结果促使开始抗结核治疗。多重实时 PCR 显示结核分枝杆菌复合群结果为阴性，非结核分枝杆菌结果为阳性。DNA 芯片检测证实该菌为分枝杆菌属的一个成员，但无法确定其种属。有趣的是，HPLC 获得的类脂酸图谱与 M. simulans 的图谱几乎重叠。分枝杆菌 16S rRNA 基因和 16S-23S 内部转录间隔区序列是独特的，与 M. riyadhense 的序列具有 100%的相似性。在查阅文献后，我们报告了首例韩国 M. riyadhense 肺部感染病例。
