Pi Zifeng, Hou Guangyue, Ai Jun, Song Fengrui, Liu Zhiqiang, Liu Shuying
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China.
Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(8):1133-9.
To establish a quick method for evaluation of the antioxidant activities based on the correlation analysis of lignans content and antioxidant activities of Schisandra chinensis fruits.
The content of five lignans components in 37 batches of S. chinensis fruits from different regions of Jilin province were measured by HPLC. Simultaneously, the antioxidant activities of the above samples were detected, such as lipid peroxidation inhibition activity in liver (LPIL), kidney (LPIK) and brain (LPIB) and the clearance rate of DPPH (CRD). Bivariate correlation analysis and stepwise regression analysis were carried out by the software of SPSS for windows 11.5.
The results of bivariate correlation analysis showed that deoxyschizandrin was negative correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrin was positive correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrol B was also positive correlation (P<0.05 or P<0.01) to the above four kinds of antioxidant activity. The results of stepwise regression analysis were mostly consistent with the bivariate correlation analysis results. For the other 10 batches of samples, the simulated antioxidant activities according to the regression equation calculated was consistent with the measured activities.
By using the bivariate correlation analysis and linear stepwise regression analysis, the bioactive components related to the antioxidant activity of S. chinensis fruits were found. Meanwhile, the antioxidant activity of samples will be inferred according to the content of Schisandra lignans.
基于五味子果实木脂素含量与抗氧化活性的相关性分析，建立一种快速评价抗氧化活性的方法。
采用高效液相色谱法测定吉林省不同地区37批次五味子果实中5种木脂素成分的含量。同时，检测上述样品的抗氧化活性，如肝脏（LPIL）、肾脏（LPIK）和大脑（LPIB）中的脂质过氧化抑制活性以及DPPH清除率（CRD）。使用SPSS for windows 11.5软件进行双变量相关性分析和逐步回归分析。
双变量相关性分析结果显示，脱氧五味子素与LPIL、LPIB、CRD活性呈负相关（P<0.01）。五味子醇甲与LPIL、LPIB、CRD活性呈正相关（P<0.01）。五味子醇乙与上述四种抗氧化活性也呈正相关（P<0.05或P<0.01）。逐步回归分析结果与双变量相关性分析结果基本一致。对于另外10批次样品，根据回归方程计算得到的模拟抗氧化活性与实测活性一致。
通过双变量相关性分析和线性逐步回归分析，发现了与五味子果实抗氧化活性相关的生物活性成分。同时，可根据五味子木脂素的含量推断样品的抗氧化活性。
