Wang Xinting, Yu Jiahui, Li Wei, Wang Chunmei, Li He, Ju Wenbo, Chen Jianguang, Sun Jinghui
College of Pharmacy, Beihua University, Jilin, 132013, P. R. China.
College of Basic Medical, Beihua University, Jilin, 132013, P. R. China.
Chem Biodivers. 2018 Jun;15(6):e1800030. doi: 10.1002/cbdv.201800030. Epub 2018 May 24.
Twenty Schisandra samples were collected from different locations. Contents of 7 lignans in the samples were determined and analyzed by HPLC method coupled with hierarchical clustering analysis (HCA) and principal component analysis (PCA), and the antioxidant capacity of Schisandra from the different locations was evaluated by reducing power, ferric thiocyanate (FTC) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay. The results showed that there was a significant difference in the content of lignans between Schisandra chinensis and Schisandra sphenanthera. The Schisandra sphenanthera samples in the southwest of China were significantly different from those from the other locations. The antioxidant capacity of Schisandra chinensis was significantly superior to that of Schisandra sphenanthera, and the main antioxidant components were schisandrol A, schisandrol B and schisandrin B based on the result of discrimination analyses. The differences in the chemical composition and antioxidant activity of lignans in Schisandra chinensis and Schisandra sphenanthera from the different locations were investigated in this study, which may provide an experimental basis for the quality control of Schisandra.
从不同地点采集了20份五味子样品。采用高效液相色谱法结合层次聚类分析（HCA）和主成分分析（PCA）对样品中7种木脂素的含量进行了测定和分析，并通过还原力、硫氰酸铁（FTC）和2,2'-二苯基-1-苦基肼（DPPH）法对不同产地五味子的抗氧化能力进行了评价。结果表明，五味子和华中五味子的木脂素含量存在显著差异。中国西南部的华中五味子样品与其他产地的样品有显著差异。五味子的抗氧化能力明显优于华中五味子，根据判别分析结果，主要抗氧化成分是五味子醇甲、五味子醇乙和五味子乙素。本研究考察了不同产地五味子和华中五味子木脂素化学成分及抗氧化活性的差异，可为五味子的质量控制提供实验依据。
