Arjomandzadegan Mohammad, Titov Leonid P, Surkova Larisa K, Farnia Parisa, Sheikholeslami Fatemeh, Owlia Parviz, Eshghinejad Arezoo, Farazi Ali Asghar, Eshrati M, Kahbazi Manijeh, Ahmadi Azam, Shojapur Mana
Research Center of Molecular Medicine, Arak University of Medical Sciences, Arak, Iran.
Tuberk Toraks. 2012;60(2):153-9. doi: 10.5578/tt.3520.
All members of the Mycobacterium tuberculosis complex were assigned to one of the three principle genetic groups based on KatG463/GyrA95 polymorphism.
A total of 202 isolates of M. tuberculosis consisting of 50 susceptible, 121 MDR (multidrug resistant) and 31 XDR (extensively drug resistant) isolated from culture-confirmed tuberculosis patients in different regions of Belarus and Iran (Tehran and Markazi province). Isolates were screened by sequencing and polymerase chain reaction restriction fragment length polymorphism (RFLP) assay, and were further divided into three principal genetic groups (PGG), based on Sreevatsan's pattern as polymorphisms in KatG463/GyrA95 codons.
Among the 104 isolates, characterized as MDR from Belarus, 57 (54.8 ± 4.8%), 30 (28.8 ± 4.43%), 17 (16.3 ± 3.6), belonged to PGG 1, 2, and 3, respectively (p< 0.05). Thirty one XDR isolates from Belarus had a similar pattern as 15 (48.4%), 12 (38.7%), 4 (12.9%) PGG 1, 2, and 3, respectively. From Iranian samples, Markazi isolates (susceptible to drugs) had a pattern as 12 (36.5%), 15 (45.5%), 3 (6%), and Tehran samples were (selected MDR): 9 (53%), 6 (35.2%), 2 (11.8%) (PGG 1, 2, and 3, respectively). In a study of tuberculosis patients, who were in prison, no relation was found between PGG and resistance to isoniazid, but most of the identified isolates belonged to PGG 1 (45.5 ± 10.9%) (p< 0.05). Overall, the group 1 isolates showed more frequency in MDR and XDR rather than susceptible strains, and there aren't any relations to geographic region.
基于KatG463/GyrA95多态性，结核分枝杆菌复合群的所有成员被归为三个主要基因群之一。
共收集了202株结核分枝杆菌分离株，这些分离株来自白俄罗斯和伊朗（德黑兰和马尔卡齐省）不同地区经培养确诊的结核病患者，其中包括50株敏感株、121株耐多药（MDR）株和31株广泛耐药（XDR）株。通过测序和聚合酶链反应-限制性片段长度多态性（RFLP）分析对分离株进行筛选，并根据Sreevatsan模式（KatG463/GyrA95密码子中的多态性）进一步分为三个主要基因群（PGG）。
在白俄罗斯的104株被鉴定为MDR的分离株中，分别有57株（54.8±4.8%）、30株（28.8±4.43%）、17株（16.3±3.6%）属于PGG 1、PGG 2和PGG 3（p<0.05）。白俄罗斯的31株XDR分离株有类似的模式，分别为15株（48.4%）、12株（38.7%）、4株（12.9%）属于PGG 1、PGG 2和PGG 3。来自伊朗样本，马尔卡齐的分离株（对药物敏感）的模式为12株（36.5%）、15株（45.5%）、3株（6%），德黑兰的样本（选择的MDR）为9株（53%）、6株（35.2%）、2株（11.8%）（分别属于PGG 1、PGG 2和PGG 3）。在一项对监狱中结核病患者的研究中，未发现PGG与对异烟肼耐药之间存在关联，但大多数鉴定出的分离株属于PGG 1（45.5±10.9%）（p<0.05）。总体而言，第1组分离株在MDR和XDR中的出现频率高于敏感株，且与地理区域无关。
