Habibi Ghasem, Arjomandzadegan Mohammad, Tayeboon Maryam, Didgar Farshideh, Sarmadian Hossein, Sadrnia Maryam, Mirhosseini Farid, Geravand Somayeh, Abdoli Mahboobeh
Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran.
Department of Biology, Payame Noor University, Tehran, Iran.
Iran J Microbiol. 2018 Oct;10(5):334-341.
Antibiotics resistance has recently increased. The aim of this study was the evaluation of antibacterial efficacy of Aloe vera carrier produced in microemulsion system in comparison with ordinary antibiotics against some Enterobacteriacea.
The aquatic extract of Aleo vera was produced by the Soxhlet method and a nonocarrier in the microemulsion system was prepared by two emulsifiers. The clinical isolates of and were obtained from patients and were identified by microbiological methods. Diffusion disk was used for evaluation of antibacterial properties in comparison with selected ordinary antibiotics. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for tested materials were determined using MTT in the Micro Broth dilution method.
The results proved that effect of carrier on studied isolates is dependent on concentration level. The inhibitory effect of carrier in concentration of 15 μg/ml by 18 mm zone of inhibition for was comparable to Ceftazidime and Cefalothin. The lowest MIC and MBC determined by the Microbroth dilution method with MTT belonged to as 0.1 and 3 μg/ml and higher concentrations belonged to at 7 and 15 μg/ml. The greatest effect of carrier of Aleo vera aquatic extract was observed for and the lowest effect belonged to and
It was concluded that the carrier of Aloe vera produced in microemulsion system was most effective and had equal effects in comparison with ordinary antibiotics against Enterobacteriacea.
近期抗生素耐药性有所增加。本研究旨在评估微乳体系中制备的芦荟载体与普通抗生素相比，对某些肠杆菌科细菌的抗菌效果。
采用索氏提取法制备芦荟水提取物，并通过两种乳化剂在微乳体系中制备无载体溶液。从患者中获取临床分离株并通过微生物学方法进行鉴定。与选定的普通抗生素相比，采用扩散纸片法评估抗菌性能。使用MTT在微量肉汤稀释法中测定受试材料的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。
结果证明载体对所研究分离株的作用取决于浓度水平。载体在浓度为15μg/ml时对[具体细菌名称1]的抑菌圈直径为18mm，其抑菌效果与头孢他啶和头孢噻吩相当。采用MTT的微量肉汤稀释法测定的最低MIC和MBC中，[具体细菌名称2]最低，分别为0.1和3μg/ml，较高浓度的是[具体细菌名称3]，为7和15μg/ml。芦荟水提取物载体对[具体细菌名称4]的作用最大，对[具体细菌名称5]和[具体细菌名称6]的作用最小。
得出结论，微乳体系中制备的芦荟载体最为有效，与普通抗生素相比，对肠杆菌科细菌具有同等效果。
