一种使用经过树脂提取的塑料包埋材料提高荧光显微镜分辨率的方法。

A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.

作者信息

Russell S D

机构信息

Department of Botany and Microbiology, University of Oklahoma, Norman 73019-0245.

出版信息

Stain Technol. 1990;65(5):259-61. doi: 10.3109/10520299009105616.

DOI:10.3109/10520299009105616

PMID:2278052

Abstract

A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.

摘要

给出了一种使用氢氧化钠、苯、丙酮和甲醇从半薄切片中提取环氧树脂的方案。对于荧光显微镜观察，此类切片似乎优于石蜡切片或未切片的材料。在ISO 3200下使用超快速胶片（例如柯达T-Max P3200），无需使用抗褪色剂且不会引入不可接受的照片颗粒尺寸，可最大程度减少褪色。