环氧树脂包埋组织切片中的自发荧光和诱导荧光。
Autofluorescence and induced fluorescence in Epon embedded tissue sections.
作者信息
del Castillo P, Molero M L, Ferrer J M, Stockert J C
出版信息
Histochemistry. 1986;85(5):439-40. doi: 10.1007/BF00982676.
PMID:3781887
Abstract
The autofluorescence of tissue components in Epon semithin sections depends on the exciting wavelength, section thickness, and the structure under observation. The degree of autofluorescence emission of a given component affects the fluorescence reaction induced by fluorochromes, a feature which must be taken into account when fluorescence microscopy is applied to the study of Epon sections.
摘要
环氧树脂半薄切片中组织成分的自发荧光取决于激发波长、切片厚度以及所观察的结构。给定成分的自发荧光发射程度会影响荧光染料诱导的荧光反应，在将荧光显微镜应用于环氧树脂切片研究时，必须考虑这一特性。
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