Stromberg P C, Grants I S, Kociba G J, Krakowka G S, Mezza L E
Department of Veterinary Pathobiology, Ohio State University, Columbus.
Vet Pathol. 1990 Nov;27(6):404-10. doi: 10.1177/030098589902700604.
A spontaneous large granular lymphocyte (LGL) leukemia was serially transplanted in 92 male F344 rats kept under standard laboratory conditions. Serial transplantation into groups of four rats each resulted in a rapid reduction in the latent period of the disease. After 23 serial transplantations, F344 rats in groups that were injected intraperitoneally with 10(7) cells died between 12 and 16 days after transplantation. At necropsy, "transplanted" rats had enlarged mesenteric lymph nodes, thymus, and spleen. Neoplastic cells were detected in the spleen on day 3 and in peripheral blood on day 6. Extreme leukocytosis with leukemia was present on day 9. Severe hemolytic anemia coincided with a sharp increase in osmotic fragility on day 12. Splenic lymphoid depletion was observed histologically and confirmed by differential cell counts of isolated spleen cells. Analysis for surface markers of splenic lymphocytes by monoclonal antibodies and flow cytometry indicated that cells with T helper/inducer phenotypes were disproportionately decreased, while the number of T suppressor cells did not significantly change. The T helper/T suppressor lymphocyte ratio (normal = 2.09 +/- 0.35) was decreased on day 9 (0.76 +/- 0.10) and day 12 (0.25 +/- 0.04). Hemolytic anemia was not related to a decrease in the number of T suppressor cells. The passaged leukemia cell model should provide investigators with an easily maintained neoplasm of short latency with which to study pathogenesis of leukemia-related disorders.
将自发性大颗粒淋巴细胞（LGL）白血病在标准实验室条件下饲养的92只雄性F344大鼠中进行连续移植。将其连续移植到每组4只大鼠中，导致疾病潜伏期迅速缩短。经过23次连续移植后，腹腔注射10(7)个细胞的组中的F344大鼠在移植后12至16天死亡。尸检时，“移植”大鼠的肠系膜淋巴结、胸腺和脾脏肿大。在第3天在脾脏中检测到肿瘤细胞，在第6天在外周血中检测到肿瘤细胞。第9天出现伴有白血病的极度白细胞增多。严重溶血性贫血与第12天渗透脆性急剧增加同时出现。通过组织学观察到脾脏淋巴样细胞耗竭，并通过对分离的脾细胞进行细胞分类计数得到证实。通过单克隆抗体和流式细胞术分析脾淋巴细胞的表面标志物表明，具有T辅助/诱导表型的细胞不成比例地减少，而T抑制细胞的数量没有显著变化。T辅助/T抑制淋巴细胞比值（正常=2.09±0.35）在第9天（0.76±0.10）和第12天（0.25±0.04）降低。溶血性贫血与T抑制细胞数量减少无关。传代白血病细胞模型应为研究人员提供一种易于维持、潜伏期短的肿瘤，用于研究白血病相关疾病的发病机制。
