利用多位点序列分析对沙眼衣原体进行高分辨率基因分型的指南。

Guidelines for high-resolution genotyping of Chlamydia trachomatis using multilocus sequence analysis.

作者信息

Christerson Linus, Herrmann Björn

机构信息

Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

出版信息

Methods Mol Biol. 2012;903:51-64. doi: 10.1007/978-1-61779-937-2_4.

DOI:10.1007/978-1-61779-937-2_4

PMID:22782811

Abstract

Chlamydia is one of the most common sexually transmitted infections worldwide and can cause ectopic pregnancies and infertility. It is therefore important to have adequate genotyping tools for investigating the spread of Chlamydia trachomatis among the population. Here, we describe a high-resolution multilocus sequence typing (MLST) system able to differentiate closely related clinical strains, which makes it ideal for short-term epidemiology and outbreak investigations. It is based on five highly variable but stable target regions which are PCR amplified and DNA sequenced.

摘要

衣原体是全球最常见的性传播感染之一，可导致宫外孕和不孕。因此，拥有足够的基因分型工具对于调查沙眼衣原体在人群中的传播至关重要。在此，我们描述了一种高分辨率多位点序列分型（MLST）系统，该系统能够区分密切相关的临床菌株，这使其非常适合短期流行病学和疫情调查。它基于五个高度可变但稳定的目标区域，通过PCR扩增和DNA测序进行分析。