Parashar Gaurav, Capalash Neena
Department of Biotechnology, Panjab University, Chandigarh, India.
Oncol Lett. 2012 May;3(5):1079-1082. doi: 10.3892/ol.2012.608. Epub 2012 Feb 13.
Promoter hypermethylation has been linked to loss of expression of tumor suppressor genes in various types of tumors. A strong reciprocal correlation between promoter hypermethylation and expression of the TIMP2 gene was observed in the Caski cell line. The TIMP2 promoter was found to be methylated within the 1919 and 1987 region (-325 to -257), relative to the transcription start site through methylation-specific PCR in the HeLa, SiHa and Caski cervical cancer cell lines. However, a reverse transcription PCR analysis of the TIMP2 gene confirmed a normal expression in the HeLa and SiHa cell lines with a high expression in the Caski cell line, indicating that expression of the TIMP2 gene is independent of methylation of CpG sites located within the -325 to -257 region of the TIMP2 promoter, relative to the transcription start site.
启动子高甲基化与多种类型肿瘤中肿瘤抑制基因表达缺失有关。在Caski细胞系中观察到TIMP2基因启动子高甲基化与表达之间存在强烈的负相关。通过甲基化特异性PCR发现，相对于转录起始位点，在HeLa、SiHa和Caski宫颈癌细胞系中，TIMP2启动子在1919至1987区域（-325至-257）内发生甲基化。然而，TIMP2基因的逆转录PCR分析证实，HeLa和SiHa细胞系中TIMP2基因表达正常，Caski细胞系中表达较高，这表明相对于转录起始位点，TIMP2基因的表达独立于TIMP2启动子-325至-257区域内CpG位点的甲基化。
