通过蛋白质表达谱鉴定胃癌患者血清中的血清淀粉样蛋白A
Identification of serum amyloid A in the serum of gastric cancer patients by protein expression profiling.
作者信息
Liu Chibo, Pan Chunqin, Shen Jianmin, Wang Haibao, Yong Liang
机构信息
Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China.
出版信息
Oncol Lett. 2012 Jun;3(6):1259-1262. doi: 10.3892/ol.2012.664. Epub 2012 Mar 30.
Abstract
The purpose of this study was to screen serum samples from gastric carcinoma patients and to determine whether serum amyloid A protein (SAA) served as a biomarker. SELDI technology was used to screen for changes in SAA levels in the serum samples. A mass cluster with a mass/charge (m/z) value between 11.1 and 11.9 kDa was identified in the serum samples from gastric carcinoma patients which was much higher than that of the control group. Furthermore, the increase in this m/z peak correlated with the severity of the cancer. High-performance liquid chromatography (HPLC) analysis confirmed that the peak was SAA1. In conclusion, this increase in SAA may be used as a potential biomarker for gastric cancer.
摘要
本研究的目的是筛查胃癌患者的血清样本，并确定血清淀粉样蛋白A（SAA）是否可作为一种生物标志物。表面增强激光解吸电离（SELDI）技术用于筛查血清样本中SAA水平的变化。在胃癌患者的血清样本中鉴定出一个质荷比（m/z）值在11.1至11.9 kDa之间的质谱峰簇，其远高于对照组。此外，该m/z峰的升高与癌症严重程度相关。高效液相色谱（HPLC）分析证实该峰为SAA1。总之，SAA的这种升高可能用作胃癌的潜在生物标志物。
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