Zhang Lixia, Pan Shiyang, Zhang Jie, Lu Lin, Xie Erfu, Ye Qin
Department of Laboratory Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China.
Clin Lab. 2012;58(5-6):563-5.
Platelet clumping caused by ethylenediamine tetraacetic acid (EDTA) and erythrocyte agglutination caused by cold agglutinins are often found in clinical findings. However, erythrocyte agglutination induced by EDTA has not been reported as yet.
Spurious low red blood cell (RBC), white blood cell (WBC), and platelet counts were observed in a patient blood sample collected in EDTA in vitro at room temperature and 37 degrees C. However, the phenomena were only observed in the sodium citrate and heparin anticoagulated blood at room temperature, but not at 37 degrees C. Both erythrocyte agglutination and platelet clumping were observed in the peripheral blood smear. These data suggest an EDTA-temperature-induced pseudohematocytopenia.
It is a very rare phenomenon to observe erythrocyte agglutination induced by EDTA and temperature.
临床检查中常发现由乙二胺四乙酸（EDTA）引起的血小板聚集和由冷凝集素引起的红细胞凝集。然而，尚未有关于EDTA诱导红细胞凝集的报道。
在室温及37℃下体外采集于EDTA的患者血样中观察到假性低红细胞（RBC）、白细胞（WBC）和血小板计数。然而，这些现象仅在室温下柠檬酸钠和肝素抗凝的血液中观察到，而在37℃时未观察到。在外周血涂片上观察到红细胞凝集和血小板聚集。这些数据提示存在EDTA温度诱导的假性血细胞减少症。
观察到EDTA和温度诱导的红细胞凝集是一种非常罕见的现象。
