Department of Characterization, Quality and Security and ‡Unit Service of Analytical Techniques, Instrumentation and Microbiology (USTA), Institute of Food Science, Technology and Nutrition (ICTAN-CSIC) , Jose Antonio Novais 10, Madrid 28040, Spain.
J Agric Food Chem. 2012 Aug 1;60(30):7489-96. doi: 10.1021/jf301547t. Epub 2012 Jul 20.
This paper deals with the impact of low temperature and high CO2 levels on flavonols, proanthocyanidins, and anthocyanins, synthesized via branched pathways from common precursors, in strawberries (Fragaria vesca L.). Flavonoids were identified with Q-TOF equipment and quantified by HPLC-quadrupole. Proanthocyanins B1 and B3 accumulated in CO2-treated strawberries, whereas in untreated (air) fruit, flavonoid production was redirected toward anthocyanin accumulation with a sharp decrease in catechin and procyanidin B3 levels. Moreover, in CO2-treated fruit, mainly in those with 20% CO2, anthocyanin accumulation did not decline. Due to its antifungal activity, catechin induction in CO2-treated strawberries could explain the capacity of high CO2 treatments to reduce fungal decay. Ascorbic acid content increased in 40% CO2-treated fruits, whereas in those treated with 20% CO2 an increase in flavonol content was observed. Despite these differences, similar antioxidant capacities were found in untreated and CO2-treated Mara de Bois strawberries.
本文研究了低温和高 CO2 水平对通过支链途径从常见前体合成的类黄酮、原花青素和花色苷的影响，这些物质在草莓（ Fragaria vesca L.）中合成。使用 Q-TOF 设备鉴定了类黄酮，并通过 HPLC-四极杆进行了定量。CO2 处理的草莓中积累了原花青素 B1 和 B3，而在未处理（空气）的果实中，类黄酮的产生被重新定向为花青素的积累，儿茶素和原花青素 B3 的水平急剧下降。此外，在 CO2 处理的果实中，特别是在 20% CO2 的果实中，花青素的积累并没有下降。由于儿茶素具有抗真菌活性，因此 CO2 处理的草莓中儿茶素的诱导可以解释高 CO2 处理降低真菌腐烂的能力。40% CO2 处理的果实中抗坏血酸含量增加，而 20% CO2 处理的果实中类黄酮含量增加。尽管存在这些差异，但未处理和 CO2 处理的 Mara de Bois 草莓具有相似的抗氧化能力。
