Institute of Agri-Food & Land Use, Queen's University Belfast, Belfast, UK.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(9):1413-24. doi: 10.1080/19440049.2012.698398. Epub 2012 Jul 12.
Mass spectrometric methods were developed and validated for the analysis in chicken muscle of a range of antibiotic growth promoters: spiramycin, tylosin, virginiamycin and bacitracin, and separately for two marker metabolites of carbadox (quinoxaline-2-carboxylic acid and 1,4-bisdesoxycarbadox), and a marker metabolite of olaquindox (3-methyl-quinoxaline-2-carboxylic acid). The use of these compounds as antibiotic growth promoters has been banned by the European Commission. This study aimed to develop methods to detect their residues in muscle samples as a means of checking for the use of these drugs during the rearing of broiler chickens. When fed growth-promoting doses for 6 days, spiramycin (31.4 µg kg(-1)), tylosin (1.0 µg kg(-1)), QCA (6.5 µg kg(-1)), DCBX (71.2 µg kg(-1)) and MQCA (0.2 µg kg(-1)) could be detected in the muscle 0 days after the withdrawal of fortified feed. Only spiramycin could consistently be detected beyond a withdrawal period of 1 day. All analytes showed stability to a commercial cooking process, therefore raw or cooked muscle could be used for monitoring purposes.
建立并验证了用于检测鸡肉中多种抗生素生长促进剂（螺旋霉素、泰乐菌素、维吉尼亚霉素和杆菌肽）以及卡巴氧两种代谢物标志物（喹喔啉-2-羧酸和 1,4-双脱氧卡巴氧）和喹乙醇代谢物标志物（3-甲基喹喔啉-2-羧酸）的质谱检测方法。欧洲委员会已禁止这些化合物作为抗生素生长促进剂使用。本研究旨在开发检测肌肉样本中这些残留物的方法，以检查在肉鸡饲养过程中是否使用这些药物。以促生长剂量连续饲喂 6 天后，停药 0 天可在肌肉中检测到螺旋霉素（31.4 µg kg(-1)）、泰乐菌素（1.0 µg kg(-1)）、QCA（6.5 µg kg(-1)）、DCBX（71.2 µg kg(-1)）和 MQCA（0.2 µg kg(-1)）。停药 1 天后仍能持续检测到螺旋霉素。所有分析物均能耐受商业烹饪过程，因此可用于监测目的的生肉或熟肉。
