Institute for Global Food Security, School of Biological Sciences, Queen's University, Belfast, UK.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(7):1270-8. doi: 10.1080/19440049.2013.801087. Epub 2013 Jun 21.
Spiramycin, tylosin, bacitracin and virginiamycin are among a group of antibiotic growth promoters that have been banned in the European Union since the 1999 Council. This was due to concerns over the development of resistant bacteria emerging between humans and animals with the threat of antibiotics no longer being able to be used effectively to treat human infections. A sensitive and fast immunochemical method is presented for the determination of these four antibiotic growth promoters simultaneously in poultry tissue. The method employs methanol extraction followed by sample clean-up by solid-phase extraction (SPE) with determination by enzyme-linked immunoabsorbant assay (ELISA). The limit of detection (LOD) was less than 1 ng g(-1) and the detection capability (CCβ) was 3 ng g(-1) or less for all four antibiotic growth promoters. Validation was completed with both raw and cooked chicken, therefore either matrix could be used for the monitoring of these banned drugs. In a feeding trial no residues of either bacitracin or virginiamycin were found in medicated birds even without a withdrawal period. In the case of tylosin and spiramycin much higher residues level were detected immunochemically than was the case by mass spectrometry.
螺旋霉素、泰乐菌素、杆菌肽和维吉尼亚霉素是一组抗生素生长促进剂，自 1999 年欧盟理事会以来已被禁止使用。这是由于担心人类和动物之间出现耐药细菌的发展，而抗生素不再能够有效地用于治疗人类感染。本文提出了一种用于同时检测家禽组织中这四种抗生素生长促进剂的灵敏、快速的免疫化学方法。该方法采用甲醇提取，然后用固相萃取（SPE）进行样品净化，最后通过酶联免疫吸附测定（ELISA）进行测定。四种抗生素生长促进剂的检测限（LOD）均小于 1ng/g，检测能力（CCβ）均为 3ng/g 或更低。该方法在生鸡肉和熟鸡肉中均进行了验证，因此可以使用任何一种基质来监测这些禁用药物。在一项喂养试验中，即使没有停药期，在用药的鸟类中也未发现杆菌肽或维吉尼亚霉素的残留。在泰乐菌素和螺旋霉素的情况下，通过免疫化学检测到的残留水平远远高于通过质谱法检测到的水平。
