Chang Chih-Chun, Chang Chieh-Yu, Huang Jiung-Pang, Hung Li-Man
"Department and Graduate Institute of Biomedical Sciences, College of Medicine Chang Gung University, Tao-Yuan 33302, Taiwan, Republic of China"
Chin J Physiol. 2012 Jun 30;55(3):192-201. doi: 10.4077/CJP.2012.BAA012.
"It has been well known that both oxidative stress and inflammatory activity play crucial roles in the pathogenesis of type 1 diabetes mellitus (T1DM). Resveratrol (RSV), a naturally occurring polyphenol found in grapes and red wine, has recently been shown to exert potent anti-diabetic, anti-oxidative and anti-inflammatory actions. In the present study, we investigated the effect of RSV on oxidative stress and inflammatory response in the liver and spleen of streptozotocin (STZ)-induced type 1 diabetic animal models. Male Long-Evans rats were injected with 65 mg/kg STZ to induce diabetes for 2 weeks, and subsequently administrated with the dosage of 0.1 or 1 mg/kg/day RSV for 7 consecutive days. Hepatic and splenic tissues were dissected for evaluation of oxidative and inflammatory stress. Oxidative stress was assessed by quantification of oxidative indicators including superoxide anion content, lipid and protein oxidative products, as well as manganese superoxide dismutase (Mn-SOD) and nitro-tyrosine protein expression levels. Inflammatory stress was evaluated by the levels of nuclear factor κB (NF-κB) and the proinflammatory cytokine tumor necrosis factorα (TNF-α), interleukin 1 β (IL-1 β ) and IL-6. The experimental results indicated that RSV significantly decreased oxidative stress (superoxide anion content, protein carbonyl level and Mn-SOD expression) in both tissues and hepatic inflammation (NF- κB and IL-1 β ), but implicated proinflammatory potential of RSV in diabetic spleen (TNF-α and IL-6). The results of this study suggest that RSV may serve as a potent antioxidant, but RSV possesses a proinflammatory potential in certain circumstances in diabetes."
众所周知，氧化应激和炎症活动在1型糖尿病（T1DM）的发病机制中都起着关键作用。白藜芦醇（RSV）是一种在葡萄和红酒中发现的天然多酚，最近已被证明具有强大的抗糖尿病、抗氧化和抗炎作用。在本研究中，我们调查了RSV对链脲佐菌素（STZ）诱导的1型糖尿病动物模型肝脏和脾脏中氧化应激和炎症反应的影响。雄性Long-Evans大鼠注射65 mg/kg STZ诱导糖尿病2周，随后连续7天给予0.1或1 mg/kg/天的RSV剂量。解剖肝脏和脾脏组织以评估氧化和炎症应激。通过量化氧化指标来评估氧化应激，这些指标包括超氧阴离子含量、脂质和蛋白质氧化产物，以及锰超氧化物歧化酶（Mn-SOD）和硝基酪氨酸蛋白表达水平。通过核因子κB（NF-κB）和促炎细胞因子肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）和IL-6的水平来评估炎症应激。实验结果表明，RSV显著降低了两种组织中的氧化应激（超氧阴离子含量、蛋白质羰基水平和Mn-SOD表达）以及肝脏炎症（NF-κB和IL-1β），但表明RSV在糖尿病脾脏中具有促炎潜力（TNF-α和IL-6）。本研究结果表明，RSV可能是一种有效的抗氧化剂，但在糖尿病的某些情况下，RSV具有促炎潜力。
