Internal Medicine Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey.
General Surgery Clinic, Umraniye Research and Training Hospital, Istanbul, Turkey.
Mol Cell Biochem. 2019 Oct;460(1-2):217-224. doi: 10.1007/s11010-019-03582-z. Epub 2019 Jul 6.
Resveratrol (RSV) is a natural polyphenolic compound having antioxidant effects. This study was designed to investigate the protective effects of resveratrol against oxidative stress in hepatic ischemia-reperfusion (I/R) injury in streptozotocin (STZ)-induced diabetic rats. STZ was injected intraperitonally (i.p.) to 18 Sprague-Dawley albino rats, which were divided into three groups, each having six rats. First group was non-treated diabetic group (D), second diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period (D + I/R), and third diabetic group was subjected to 30 min of hepatic ischemia followed by a 45-min reperfusion period and treated with 20 mg/kg/day oral RSV before 30 min I/R injury (D + I/R + RSV). At the end of the experimental period, animals were decapitated, and blood samples were collected to determine tissue tumor necrosis factor-α (TNF-α) levels. Liver and lung tissue samples were obtained for the evaluation of biochemical parameters including malondialdehyde (MDA) and glutathione (GSH) levels and histopathological examinations. Compared to control, I/R injury resulted in decreases in GSH levels and increases in MDA levels. Tissue TNF-α levels were also increased in the D + I/R group compared to D group. Treatment with RSV prevented the alterations on biochemical parameters and histopathological changes induced by I/R. We demonstrate that in diabetic rats, hepatic I/R injury is associated with an augmented inflammatory response and oxidative stress, while RSV pre-treatment significantly decreased these responses. Larger clinical studies are desirable to determine the exact role(s) of RSV on hepatic I/R injury among diabetic subjects.
白藜芦醇（RSV）是一种具有抗氧化作用的天然多酚化合物。本研究旨在探讨白藜芦醇对链脲佐菌素（STZ）诱导的糖尿病大鼠肝缺血再灌注（I/R）损伤中氧化应激的保护作用。STZ 经腹腔注射（i.p.）至 18 只 Sprague-Dawley 白化大鼠，将其分为三组，每组 6 只。第一组为未治疗的糖尿病组（D），第二组糖尿病组接受 30 分钟肝缺血，然后进行 45 分钟再灌注期（D+I/R），第三组糖尿病组接受 30 分钟肝缺血，然后进行 45 分钟再灌注期，并在 30 分钟 I/R 损伤前给予 20mg/kg/天口服 RSV（D+I/R+RSV）。在实验期末，动物断头采血，收集血液样本以测定组织肿瘤坏死因子-α（TNF-α）水平。获取肝和肺组织样本，评估生化参数，包括丙二醛（MDA）和谷胱甘肽（GSH）水平，并进行组织病理学检查。与对照组相比，I/R 损伤导致 GSH 水平降低和 MDA 水平升高。与 D 组相比，D+I/R 组的组织 TNF-α 水平也升高。RSV 治疗可防止 I/R 引起的生化参数和组织病理学变化。我们证明，在糖尿病大鼠中，肝 I/R 损伤与增强的炎症反应和氧化应激有关，而 RSV 预处理可显著降低这些反应。需要更大的临床研究来确定 RSV 在糖尿病患者肝 I/R 损伤中的确切作用。
