Haq Zulfqarul, Ahmad Syed Mudasir, Bashir Ishrat, Dar Mashooq Ahmad, Saleem Afnan, Khan Azmat Alam, Yatoo Mohammad Iqbal, Mir Shabir, Rastogi Ankur, Hussain Mohd Isfaqul, Shah Riaz A, Bhat Basharat
Division of Livestock Production and Management, Faculty of Veterinary Sciences & Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, India.
Division of Animal Biotechnology, FVSc & AH, Shuhama, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, India.
Front Vet Sci. 2022 Jun 2;9:866614. doi: 10.3389/fvets.2022.866614. eCollection 2022.
serovar () is the leading cause of foodborne illness. Since continues to have a detrimental effect on public health, there is an ongoing need to develop more advanced methods for combating Salmonellosis in foods before they reach consumers. In addition, the quest for alternative natural products has recently intensified due to increasingly stringent regulations regarding the use of antibiotics as growth promoters and consumer demand for antibiotic-free poultry products. This study evaluated the effect of Ajwain extract (AJE) on immune response and antioxidant status in broiler chicks challenged with . The chicks were infected with and were divided into the different groups, except for the control group (CON). The challenged chicks received different treatments with 3 × 10 colony-forming unit (CFU) Acipro-WS probiotic (PRO), 200 mg/kg Ajwain extract (AJE), 200 mg/100 kg of enrofloxacin (ENR), and a combination of 3 × 10 CFU Acipro-WS probiotic and 200 mg/kg Ajwain extract (COM). Five days posttreatment, the tissue samples (liver and spleen) were analyzed. The results showed that basal diet supplemented with Ajwain extract (AJE) and a combination of probiotic and Ajwain extract (COM) significantly ( < 0.0.5) reduced the cytokine expression in broiler chicks challenged with . Our findings suggest that AJE can clear the bacterial infection, improve antioxidant status, and suppress the inflammation response. Additionally, AJE supplementation significantly mitigated the -induced increase in the interleukin-6 (IL-6) (liver and spleen), interleukin-8 (IL-8) (liver and spleen), interleukin-17A (IL-17A) (liver and spleen), and inducible nitric oxide (iNOS) (spleen and liver) levels ( < 0.05). We conclude that Ajwain is an efficient feed additive with antioxidant and anti-inflammatory properties. The interaction networks developed in this study provide a novel lead that could be targeted for anti-inflammatory and antioxidant properties.
血清型（ ）是食源性疾病的主要病因。由于 继续对公众健康产生不利影响，因此持续需要开发更先进的方法，以便在食品到达消费者之前对抗其中的沙门氏菌病。此外，由于关于使用抗生素作为生长促进剂的法规日益严格以及消费者对抗生素无残留家禽产品的需求，最近对替代天然产品的探索有所加强。本研究评估了阿育吠陀提取物（AJE）对受到 攻击的肉鸡雏鸡免疫反应和抗氧化状态的影响。雏鸡感染 后，除对照组（CON）外，分为不同组。受到攻击的雏鸡接受不同处理，分别为3×10菌落形成单位（CFU）的阿西普罗 - WS益生菌（PRO）、200毫克/千克阿育吠陀提取物（AJE）、200毫克/100千克恩诺沙星（ENR），以及3×10 CFU阿西普罗 - WS益生菌和200毫克/千克阿育吠陀提取物的组合（COM）。处理后五天，分析组织样本（肝脏和脾脏）。结果表明，补充阿育吠陀提取物（AJE）以及益生菌和阿育吠陀提取物的组合（COM）的基础日粮显著（ < 0.0.5）降低了受到 攻击的肉鸡雏鸡中的细胞因子表达。我们的研究结果表明，AJE可以清除细菌感染、改善抗氧化状态并抑制炎症反应。此外，补充AJE显著减轻了 诱导的白细胞介素 - 6（IL - 6）（肝脏和脾脏）、白细胞介素 - 8（IL - 8）（肝脏和脾脏）、白细胞介素 - 17A（IL - 17A）（肝脏和脾脏）以及诱导型一氧化氮合酶（iNOS）（脾脏和肝脏）水平的升高（ < 0.05）。我们得出结论，阿育吠陀是一种具有抗氧化和抗炎特性的高效饲料添加剂。本研究中开发的相互作用网络提供了一个可针对抗炎和抗氧化特性的新线索。
