Department of Oral Surgery, Medical University of Vienna, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Clin Oral Implants Res. 2013 Mar;24(3):285-9. doi: 10.1111/j.1600-0501.2012.02533.x. Epub 2012 Jul 12.
Osteocytes, the most common cells of the bone, are buried in lacunae. Density and area of the osteocyte lacunae change with increasing maturation of the newly formed bone. Evaluation of osteocyte lacunae can therefore provide insights into the process of graft consolidation.
Here, we determined the osteocyte lacunar density (number of osteocyte lacunae per bone area; N.Ot/BAr) and the osteocyte lacunar area in μm(2) (Lac.Ar) in histological specimens 6 and 12 weeks after the sinuses of 10 minipigs were augmented with Bio-Oss(®) , a deproteinized bovine bone mineral, and Ostim(®) , an aqueous paste of synthetic nanoparticular hydroxyapatite. The region of interest was defined by the following criteria: (i) >1 mm from the host bone, (ii) >0.5 mm from the sinus mucosa, (iii) minimum area of 0.2 mm(2) , and (iv) bone tissue spanning at least two bone substitute particles.
The overall osteocyte lacunar density was significantly higher in the Bio-Oss(®) group than in the Ostim(®) group and decreased during the observation period at a similar range in both groups. The osteocyte lacunar area was smaller in the Bio-Oss(®) group than the Ostim(®) group but there was no significant change within the groups over time.
These results suggest that bone substitutes affect the osteocyte lacunar density and the osteocyte lacunar area in the newly formed bone within the augmented sinus in this particular model situation. These measures can provide insights into the maturation of newly formed bone in the augmented sinus.
成骨细胞是骨骼中最常见的细胞，它们被埋藏在陷窝中。随着新形成骨的成熟度增加，骨细胞陷窝的密度和面积会发生变化。因此，评估骨细胞陷窝可以深入了解移植物整合的过程。
本研究在 10 只小型猪的鼻窦中分别用 Bio-Oss®（一种去蛋白牛骨矿物质）和 Ostim®（一种合成纳米级羟基磷灰石的水性糊剂）进行骨增量 6 和 12 周后，通过组织学标本确定了骨细胞陷窝密度（每骨面积的骨细胞陷窝数量；N.Ot/BAr）和 μm²（Lac.Ar）的骨细胞陷窝面积。感兴趣区域通过以下标准定义：（i）距宿主骨>1mm，（ii）距窦粘膜>0.5mm，（iii）最小面积 0.2mm²，（iv）跨越至少两个骨替代物颗粒的骨组织。
总体而言，Bio-Oss®组的骨细胞陷窝密度明显高于 Ostim®组，并且在两个组中，观察期间以相似的范围降低。Bio-Oss®组的骨细胞陷窝面积小于 Ostim®组，但两组内随时间无明显变化。
这些结果表明，在这种特定的模型情况下，骨替代物会影响新形成的窦内骨中的骨细胞陷窝密度和骨细胞陷窝面积。这些措施可以深入了解新形成的窦内骨的成熟度。
