Busenlechner Dieter, Huber Christian D, Vasak Christoph, Dobsak Antonia, Gruber Reinhard, Watzek Georg
Department of Oral Surgery, Medical University of Vienna, Vienna, Austria.
Clin Oral Implants Res. 2009 Oct;20(10):1078-83. doi: 10.1111/j.1600-0501.2009.01733.x. Epub 2009 Jun 10.
Graft consolidation follows a gradient that reflects the properties of bone substitutes at sites of sinus augmentation. Here we present an analytical method to investigate the process of graft consolidation taking the distance from the maxillary host bone into account.
We therefore evaluated histological specimens, 6 and 12 weeks after the sinus of minipigs was augmented with Bio-Oss((R)), a deproteinized bovine bone mineral, and Ostim((R)), an aqueous paste of synthetic nanoparticular hydroxyapatite. A curve was drawn that represents the changes in histomorphometric parameters within a given distance from the maxillary host bone.
Based on this curve, three regions of interest were defined: R1 (0-1 mm) the bridging distance where new bone is laid onto the host bone, R2 (2-3 mm) a region of osteoconduction where new bone exclusively grows on the biomaterial, R3 (4-5 mm) and a region of osteoconduction where bone formation has reached its maximal extension. Qualitative and quantitative analysis of the three regions can reveal differences in graft consolidation, depending on the bone substitutes and the observation period [Bone volume (BV) per tissue volume after 6 weeks: R1: 19+/-8.4% for Bio-Oss((R)) and 42.9+/-13.2% for Ostim((R)) (P=0.03), R2: 3+/-2.4% for Bio-Oss((R)) and 14.7+/-9.5% for Ostim((R)) (P=0.03), R3: 5+/-4.1% for Bio-Oss((R)) and 5.3+/-5.3% for Ostim((R)) (P=0.86). BV per tissue volume after 12 weeks: R1: 38.0+/-13.3% for Bio-Oss((R)) and 53.3+/-6.6 for Ostim((R)) (P=0.04), R2: 14+/-12.2 for Bio-Oss((R)) and 26.4+/-11 for Ostim((R)) (P=0.18), R3: 6.6+/-7 for Bio-Oss((R)) and 10.7+/-5.8 for Ostim((R)) (P=0.32) after 12 weeks].
Based on the graft consolidation gradient, the impact of bone substitutes to modulate the process of bone formation and the kinetic of degradation within a distinct region of the augmented sinus can be investigated.
植骨融合遵循一种梯度变化，该梯度反映了鼻窦增高部位骨替代物的特性。在此，我们提出一种分析方法，以研究考虑到与上颌宿主骨距离的植骨融合过程。
因此，我们评估了小型猪鼻窦用Bio-Oss（一种脱蛋白牛骨矿物质）和Ostim（一种合成纳米颗粒羟基磷灰石水糊剂）增高6周和12周后的组织学标本。绘制了一条曲线，该曲线表示距上颌宿主骨给定距离内组织形态计量学参数的变化。
基于此曲线，定义了三个感兴趣区域：R1（0 - 1毫米）为新骨沉积在上颌宿主骨上的桥接距离，R2（2 - 3毫米）为骨传导区域，新骨仅在生物材料上生长，R3（4 - 5毫米）为骨传导区域，骨形成已达到最大范围。对这三个区域的定性和定量分析可以揭示植骨融合的差异，这取决于骨替代物和观察期[6周后每组织体积的骨体积（BV）：R1：Bio-Oss为19±8.4%，Ostim为42.9±13.2%（P = 0.03）；R2：Bio-Oss为3±2.4%，Ostim为14.7±9.5%（P = 0.03）；R3：Bio-Oss为5±4.1%，Ostim为5.3±5.3%（P = 0.86）。12周后每组织体积的BV：R1：Bio-Oss为38.0±13.3%，Ostim为53.3±6.6（P = 0.04）；R2：Bio-Oss为14±12.2，Ostim为26.4±11（P = 0.18）；12周后R3：Bio-Oss为6.6±7，Ostim为10.7±5.8（P = 0.32）]。
基于植骨融合梯度，可以研究骨替代物对调节骨形成过程以及在增高鼻窦特定区域内降解动力学的影响。
