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应用生物标志物响应评估中国胶州湾贝类(栉孔扇贝)中的金属和多环芳烃污染。

Application of the biomarker responses in scallop (Chlamys farreri) to assess metals and PAHs pollution in Jiaozhou Bay, China.

机构信息

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.

出版信息

Mar Environ Res. 2012 Sep;80:38-45. doi: 10.1016/j.marenvres.2012.06.008. Epub 2012 Jun 23.

DOI:10.1016/j.marenvres.2012.06.008
PMID:22784563
Abstract

Scallops (Chlamys farreri) were collected at three sites in the Jiaozhou Bay, China in April and July 2007. The responses of biomarkers were evaluated for assessment of physiological status of scallops and pollution in the areas. Compared with site S1, the activity of aryl hydrocarbon hydroxylase was induced notably (P < 0.05) and glutathione S-transferase activity was inhibited significantly (P < 0.05) in S2 and S3. The levels of DNA alkaline unwinding, DNA protein crosslinks, protein carbonyl and lipid peroxidation were tested and indicated the oxidative stress situation of scallops. There was significant difference of biomarker levels between sampling seasons (P < 0.05), and between digestive gland and gill (P < 0.05). The results provided the reference data for multiple-pollution assessment in the marine environment and indicated that tissue type and seasons affect biomarkers, therefore these factors should be taken into consideration when biomarkers are used for environmental assessment purposes.

摘要

2007 年 4 月和 7 月,在中国胶州湾的三个地点采集了扇贝(Chlamys farreri)。评估了生物标志物的反应,以评估扇贝的生理状况和该地区的污染情况。与 S1 点相比,S2 和 S3 点的芳烃羟化酶活性明显升高(P < 0.05),谷胱甘肽 S-转移酶活性显著抑制(P < 0.05)。测试了 DNA 碱性解旋、DNA 蛋白质交联、蛋白质羰基和脂质过氧化水平,表明扇贝处于氧化应激状态。生物标志物水平在采样季节之间(P < 0.05)和消化腺和鳃之间(P < 0.05)存在显著差异。结果为海洋环境的多污染评估提供了参考数据,并表明组织类型和季节会影响生物标志物,因此在将生物标志物用于环境评估目的时,应考虑这些因素。

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