Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Weigang Road 1, Nanjing 210095, China.
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
Biomolecules. 2019 Jul 27;9(8):310. doi: 10.3390/biom9080310.
Thiazolidinedione 49 (TD), a newly synthesized algicide, shows strong toxicity at low concentrations of 0.1-2.0 μM. However, its potential effects on non-target species at the transcript level were not investigated. Differentially expressed genes (DEGs) in the gills of the bay scallop, , were accessed after treatment with 0.68 μM TD for up to 48 h. Following exposure, it was observed that 5214 genes were upregulated and 3497 were downregulated. Functional enrichment analysis revealed that the apoptosis pathway was activated. The extrinsic apoptosis pathway was activated and the survival factors related pathway was suppressed. Furthermore, gene expressions related to ATP-binding cassette, nuclear factor erythroid 2-related factor, B cell lymphoma-2 family protein, glutathione reductase, glutathione peroxidase, catalase, NADPH2:quinone reductase, and superoxide dismutase were decreased. Conversely, gene expressions related to FAS-associated death domain protein, glutathione -transferase, caspase 6, 8, cytochrome P450 1A1, and 2C8 were increased. These results comprehensively demonstrated the toxicity of the novel algicide TD, and should draw the attention of researchers to the importance of analyzing the potential impact of chemical compounds as algicides to control the proliferation of harmful algae, due to the secondary pollution caused by their application.
噻唑烷二酮 49（TD）是一种新合成的杀藻剂，在 0.1-2.0 μM 的低浓度下显示出很强的毒性。然而，其在转录水平上对非靶标物种的潜在影响尚未被研究。用 0.68 μM TD 处理贻贝鳃 48 小时后，检测到差异表达基因（DEGs）。暴露后，观察到 5214 个基因上调，3497 个基因下调。功能富集分析表明凋亡途径被激活。外源性凋亡途径被激活，生存因子相关途径被抑制。此外，与 ABC 转运蛋白、核因子红细胞 2 相关因子、B 细胞淋巴瘤-2 家族蛋白、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、过氧化氢酶、NADPH2：醌还原酶和超氧化物歧化酶相关的基因表达减少。相反，与 FAS 相关死亡域蛋白、谷胱甘肽转移酶、半胱氨酸蛋白酶 6、8、细胞色素 P450 1A1 和 2C8 相关的基因表达增加。这些结果全面证明了新型杀藻剂 TD 的毒性，应引起研究人员的重视，即需要分析化学化合物作为杀藻剂控制有害藻类增殖的潜在影响，因为它们的应用会造成二次污染。
