Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, Weigang Road 1, Nanjing 210095, China.
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea.
Biomolecules. 2018 Nov 6;8(4):139. doi: 10.3390/biom8040139.
Palmitoleic acid (PA) is an effective algicide against . However, the toxicological mechanism of PA exposure is unclear. The transcript abundance and differentially expressed genes (DEGs) in gills of bay scallop were investigated following 80 mg/L PA exposure up to 48 h using the Illumina HiSeq 4000 deep-sequencing platform with the recommended read length of 100 bp. De novo assembly of paired-end reads yielded 62,099 unigenes; 5414 genes were identified as being significantly increased, and 4452 were decreased. Based on gene ontology classification and enrichment analysis, the 'cellular process', 'metabolic process', 'response to stimulus', and 'catalytic process' with particularly high functional enrichment were revealed. The DEGs, which are related to detoxification and immune responses, revealed that acid phosphatase, fibrinogen C domain-containing protein, cyclic AMP-responsive element-binding protein, lutathione reductase, ATP-binding cassette, nuclear factor erythroid 2-related factor, NADPH2:quinone reductase, and cytochrome P450 4F22, 4B1, and 2C8-related gene expression decreased. In contrast, some genes related to glutathione -transferase, C-type lectin, superoxide dismutase, toll-like receptors, and cytochrome P450 2C14, 2U1, 3A24 and 4A2 increased. The results of current research will be a valuable resource for the investigation of gene expression stimulated by PA, and will help understanding of the molecular mechanisms underlying the scallops' response to PA exposure.
棕榈油酸（PA）是一种有效的藻类杀生物剂，可有效对抗赤潮。然而，PA 暴露的毒理学机制尚不清楚。本研究采用 Illumina HiSeq 4000 高通量测序平台，以 100bp 的推荐读长对经 80mg/L PA 暴露 48 小时的海湾扇贝鳃组织进行转录组测序，共获得 62099 条 unigenes；其中有 5414 个基因表达显著上调，4452 个基因表达显著下调。根据基因本体论（GO）分类和富集分析，结果显示“细胞过程”、“代谢过程”、“对刺激的反应”和“催化活性”等功能具有较高的富集度。与解毒和免疫反应相关的差异表达基因（DEGs）显示，酸性磷酸酶、纤维蛋白原 C 结构域蛋白、环磷酸腺苷反应元件结合蛋白、谷胱甘肽还原酶、ATP 结合盒、红细胞系 2 相关因子、NADPH2：醌还原酶和细胞色素 P450 4F22、4B1 和 2C8 相关基因表达降低。相反，一些与谷胱甘肽转移酶、C 型凝集素、超氧化物歧化酶、Toll 样受体和细胞色素 P450 2C14、2U1、3A24 和 4A2 相关的基因表达增加。本研究的结果将为研究 PA 刺激基因表达提供有价值的资源，并有助于了解扇贝对 PA 暴露的分子机制。
