Department of Civil and Environmental Engineering, University of California, Davis, CA 95616, USA.
Bioresour Technol. 2012 Sep;120:285-9. doi: 10.1016/j.biortech.2012.06.024. Epub 2012 Jun 16.
Polyhydroxyalkanoate (PHA) was produced in bench-scale sequencing batch reactors (SBRs) fed olive pomace fermentate containing a mixed microbial consortium. Initial anaerobic fermentation in a sequencing batch fermentor (SBF) produced soluble carbon compounds, mainly organic acids. SBF effluent was centrifuged, removing solids, and fed into a SBR where intracellular PHA was produced. Buffer pre-treatment of diluted olive pomace increased organic acid production 75% in SBF fermentate over no pre-treatment. Hydraulic retention time (HRT), solids retention time (SRT), pomace concentration/dilution, and aerobic operation vs. anoxic/oxic cycling were studied to improve PHA formation. Maximum %PHA achieved was 39% (on a dry-weight cell basis), and the highest volumetric productivity was 0.042 g PHA/L-day under fully aerobic conditions. The highest PHA conversion yield was 0.3625 g PHA/L fermentate.
聚羟基烷酸酯（PHA）在中试规模的序批式反应器（SBR）中生产，采用橄榄渣发酵液作为底物，其中含有混合微生物群落。序批式发酵器（SBF）中的初始厌氧发酵产生了可溶性碳化合物，主要是有机酸。SBF 流出物经过离心，去除固体，然后输送到 SBR 中，在那里产生细胞内 PHA。缓冲液预处理稀释的橄榄渣，使 SBF 发酵液中的有机酸产量比未经预处理时增加了 75%。研究了水力停留时间（HRT）、固体停留时间（SRT）、渣浓度/稀释度、好氧操作与缺氧/好氧循环，以提高 PHA 的形成。最大的 PHA 含量达到 39%（以干重细胞为基础），在完全好氧条件下，最大的比生长产率为 0.042 gPHA/L-天。最高的 PHA 转化率为 0.3625 gPHA/L 发酵液。
