Yranzo-Volonté N, Riera C M
Inmunologia y Serologia, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
Am J Reprod Immunol. 1990 Aug;23(4):99-103. doi: 10.1111/j.1600-0897.1990.tb00680.x.
Adult female rats were immunized with 5 mg or 25 mg of modified rat male accessory glands (MRAG) incorporated to complete Freund's adjuvant (CFA) before, during, and after pregnancy. The mothers and litters were exchanged between the experimental and normal groups. The offspring were brought up to 20 days of age and immunized with 5 mg of MRAG-CFA and 5 mg of human serum albumin (HSA)-CFA. Anti-MRAG antibodies were detected in the offspring brought up by the immunized mothers and the titers were similar to those of the foster mothers whereas in the offspring of the experimental group fostered by normal mothers antibodies to MRAG were not detected. The DTH performed in the offspring 13 days after the immunization was significantly diminished in male and female offspring from the 5 mg and 25 mg experimental group fostered by normal mothers (P less than 0.0005 for all groups). Similar results were found when the offspring from normal mothers were suckled by mothers immunized with MRAG-CFA. To assess whether MRAG or HSA administered to female rats reached the offspring via the placenta or the milk, female rats were immunized with 3 mg of 125I-MRAG-CFA or with 3 mg of 125I-HSA-CFA. When radioactivity was measured in neonates (n = 11) that were suckled by the 125I-MRAG-CFA immunized mothers, the specific activity was 116 in stomach (0.4 micrograms of MRAG) and 940 in the total organs (3.8 micrograms of MRAG).(ABSTRACT TRUNCATED AT 250 WORDS)
成年雌性大鼠在怀孕前、怀孕中和怀孕后，用5毫克或25毫克掺入完全弗氏佐剂（CFA）的改良大鼠雄性附属性腺（MRAG）进行免疫。母鼠和幼崽在实验组和正常组之间进行交换。将后代饲养至20日龄，并用5毫克MRAG - CFA和5毫克人血清白蛋白（HSA） - CFA进行免疫。在由免疫母鼠抚养的后代中检测到抗MRAG抗体，其滴度与代孕母鼠相似，而在由正常母鼠抚养的实验组后代中未检测到抗MRAG抗体。在免疫后13天对后代进行的迟发型超敏反应（DTH）中，由正常母鼠抚养的5毫克和25毫克实验组的雄性和雌性后代中，该反应明显减弱（所有组P均小于0.0005）。当正常母鼠的后代由用MRAG - CFA免疫的母鼠哺乳时，也发现了类似结果。为了评估给雌性大鼠注射的MRAG或HSA是否通过胎盘或乳汁到达后代，用3毫克125I - MRAG - CFA或3毫克125I - HSA - CFA对雌性大鼠进行免疫。当对由125I - MRAG - CFA免疫的母鼠哺乳的新生儿（n = 11）进行放射性测量时，胃中的比活度为116（含0.4微克MRAG），全身器官中的比活度为940（含3.8微克MRAG）。（摘要截断于250字）
