Ferro M E, Romero-Piffiguer M, Riera C M
Inmunologia, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, Argentina.
Autoimmunity. 1989;5(1-2):25-35. doi: 10.3109/08916938909029140.
The present paper describes a mechanism responsible for the induction of inducer-phase suppressor cells effective to suppress the autoimmune response to rat male accessory glands (RAG). In fact, we reported here that marked suppression of delayed type hypersensitivity (DTH) reaction and humoral response to chemically modified rat male accessory glands (MRAG) can be obtained when previously to be immunized with MRAG in complete Freund's adjuvant (CFA) syngeneic rats were pretreated with peritoneal cells (PC) coupled with a purified fraction of RAG (containing the autoantigen). The involvement of MRAG-specific inducer-phase suppressor cells was demonstrated by adoptive transfer experiments of spleen mononuclear cells from unresponsive donors to normal syngeneic rats 24 h prior to immunization of the recipients with MRAG-CFA. The PC used to treat the animals show a large proportion of non-specific-esterase positive, Ox-41 bearing macrophage-like cells. Moreover, the antigen-coupled PC able to trigger the suppressor cells showed the presence of the autoantigen of RAG on their surface. The role of the antigen presenting cells in the induction of MRAG-specific inducer-phase suppressor cells is discussed.
本文描述了一种机制，该机制负责诱导对抑制大鼠雄性附属腺（RAG）自身免疫反应有效的诱导期抑制细胞。事实上，我们在此报告，当用化学修饰的大鼠雄性附属腺（MRAG）在完全弗氏佐剂（CFA）中对同基因大鼠进行免疫之前，先用与RAG纯化组分（含自身抗原）偶联的腹腔细胞（PC）进行预处理时，可显著抑制迟发型超敏反应（DTH）和对化学修饰的大鼠雄性附属腺（MRAG）的体液反应。在受体用MRAG - CFA免疫前24小时，将无反应供体的脾单核细胞过继转移至正常同基因大鼠，通过该实验证明了MRAG特异性诱导期抑制细胞的参与。用于治疗动物的PC显示出很大比例的非特异性酯酶阳性、带有Ox - 41的巨噬细胞样细胞。此外，能够触发抑制细胞的抗原偶联PC在其表面显示出RAG自身抗原的存在。本文讨论了抗原呈递细胞在诱导MRAG特异性诱导期抑制细胞中的作用。
