Ferrari A C, Seuanez H N, Hanash S M, Atweh G F
Division of Hematology and Oncology, VA Medical Center, Brooklyn, New York 11209.
Genes Chromosomes Cancer. 1990 Jul;2(2):125-9. doi: 10.1002/gcc.2870020208.
The cytosolic protein p18 which is expressed in increased amounts in acute leukemia cells is variably phosphorylated as a function of growth and differentiation. Proteins with identical amino acid sequence were independently found to be highly expressed in normal brain tissue and neuroendocrine tumor cells. Here we described the mapping of the recently cloned p18 gene to chromosome 1, band p35-36.1 by Southern blot analysis of human-rodent somatic cell hybrid DNA and by chromosome in situ hybridization using a p18 genomic probe. This region of the distal short arm of chromosome 1 is a frequent site of deletions or loss of heterozygosity in tumors derived from neural crest cells, particularly neuroblastomas and melanomas. The high levels of expression of p18 in brain and neuroendocrine tumor cells, its possible role in growth regulation, and its chromosomal location in a region frequently deleted in neuroectodermal tumors suggest that this gene may be involved in common genetic events occurring in these tumors.
胞质蛋白p18在急性白血病细胞中表达量增加，其磷酸化程度随生长和分化而变化。具有相同氨基酸序列的蛋白质被独立发现于正常脑组织和神经内分泌肿瘤细胞中高表达。在此，我们通过对人-啮齿类体细胞杂种DNA进行Southern印迹分析以及使用p18基因组探针进行染色体原位杂交，将最近克隆的p18基因定位到1号染色体p35 - 36.1带。1号染色体短臂远端的这个区域是源自神经嵴细胞的肿瘤，特别是神经母细胞瘤和黑色素瘤中缺失或杂合性丢失的常见位点。p18在脑和神经内分泌肿瘤细胞中的高表达水平、其在生长调节中的可能作用以及其在神经外胚层肿瘤中经常缺失区域的染色体定位表明，该基因可能参与了这些肿瘤中发生的常见遗传事件。
