Benchimol S, Lamb P, Crawford L V, Sheer D, Shows T B, Bruns G A, Peacock J
Somat Cell Mol Genet. 1985 Sep;11(5):505-10. doi: 10.1007/BF01534845.
The human gene for the transformation-associated p53 phosphoprotein (P53) was assigned to the short arm of chromosome 17 using human-rodent somatic cell hybrids and Southern filter hybridization of cell hybrid DNA. The filters were hybridized to radiolabeled DNA from a genomic clone which contained P53 nucleotide sequences. Hybridization of the probe to a 2.5-kb human DNA fragment in HindIII-digested DNA was used to identify the human P53 gene.
利用人-啮齿动物体细胞杂种以及细胞杂种DNA的Southern印迹杂交技术，将与转化相关的p53磷蛋白（P53）的人类基因定位于17号染色体的短臂上。印迹膜与来自一个包含P53核苷酸序列的基因组克隆的放射性标记DNA杂交。用该探针与经HindIII酶切的DNA中的一个2.5kb人类DNA片段杂交，以此来鉴定人类P53基因。
