Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, United States.
Curr Opin Virol. 2012 Aug;2(4):380-8. doi: 10.1016/j.coviro.2012.06.003. Epub 2012 Jul 11.
The replication of rotavirus is a complex process that is orchestrated by an exquisite interplay between the rotavirus non-structural and structural proteins. Subsequent to particle entry and genome transcription, the non-structural proteins coordinate and regulate viral mRNA translation and the formation of electron-dense viroplasms that serve as exclusive compartments for genome replication, genome encapsidation and capsid assembly. In addition, non-structural proteins are involved in antagonizing the antiviral host response and in subverting important cellular processes to enable successful virus replication. Although far from complete, new structural studies, together with functional studies, provide substantial insight into how the non-structural proteins coordinate rotavirus replication. This brief review highlights our current knowledge of the structure-function relationships of the rotavirus non-structural proteins, as well as fascinating questions that remain to be understood.
轮状病毒的复制是一个复杂的过程，由轮状病毒的非结构蛋白和结构蛋白之间的精巧相互作用来协调。在颗粒进入和基因组转录之后，非结构蛋白协调和调节病毒 mRNA 的翻译，并形成电子致密的 viroplasms，作为基因组复制、基因组包裹和衣壳组装的专用隔间。此外，非结构蛋白还参与拮抗抗病毒宿主反应，并颠覆重要的细胞过程，以实现病毒的成功复制。尽管还远未完成，但新的结构研究，以及功能研究，为我们提供了大量关于非结构蛋白如何协调轮状病毒复制的见解。这篇简短的综述强调了我们目前对轮状病毒非结构蛋白的结构-功能关系的了解，以及仍有待理解的迷人问题。
