Bismarck Doris, Schütze Nicole, Moore Peter, Büttner Mathias, Alber Gottfried, Buttlar Heiner v
Institute of Immunology, College of Veterinary Medicine, University of Leipzig, An den Tierkliniken 11, 04103 Leipzig, Germany.
Vet Immunol Immunopathol. 2012 Oct 15;149(3-4):157-66. doi: 10.1016/j.vetimm.2012.06.014. Epub 2012 Jun 18.
In dogs a CD4(+)CD8(+) double positive T cell subpopulation exists that has not been phenotypically defined yet. We demonstrate that canine CD4(+)CD8(+) T cells are mature CD1a(-) and TCRαβ(+) T cells. To analyse the activation potential of CD4(+)CD8(+) T cells, PBMC from dogs vaccinated against canine distemper virus (CDV) were re-stimulated with CDV. Upon antigen-specific stimulation, the CD4(+)CD8(+) T cell fraction increases and consists nearly exclusively of proliferated cells. Similarly, other features of activated effector/memory T cells such as up-regulation of CD25 and MHC-II as well as down-regulation of CD62L (L-selectin) were observed in CD4(+)CD8(+) T cells after stimulation. Canine CD4(+)CD8(+) T cells are less abundant, but more heterogeneous than porcine ones, comprising a small proportion expressing the β chain of CD8 in addition to the CD8α chain, like human CD4(+)CD8(+) T cells. In summary, this analysis provides the basis for functional characterisation of the in vivo relevance of CD4(+)CD8(+) T cells in T-cell mediated immunity.
在犬类中存在一个尚未进行表型定义的CD4(+)CD8(+)双阳性T细胞亚群。我们证明犬类CD4(+)CD8(+) T细胞是成熟的CD1a(-)和TCRαβ(+) T细胞。为了分析CD4(+)CD8(+) T细胞的激活潜能，用犬瘟热病毒（CDV）对犬进行疫苗接种后，采集其外周血单核细胞（PBMC）并用CDV进行再次刺激。在抗原特异性刺激后，CD4(+)CD8(+) T细胞比例增加，且几乎完全由增殖细胞组成。同样，在刺激后的CD4(+)CD8(+) T细胞中观察到了活化效应/记忆T细胞的其他特征，如CD25和MHC-II的上调以及CD62L（L-选择素）的下调。犬类CD4(+)CD8(+) T细胞比猪的CD4(+)CD8(+) T细胞数量少，但异质性更高，其中一小部分除了表达CD8α链外，还表达CD8的β链，类似于人类CD4(+)CD8(+) T细胞。总之，该分析为CD4(+)CD8(+) T细胞在T细胞介导的免疫中体内相关性的功能表征提供了基础。
