抗氧化酶基因型与活性和肾功能之间的关系：一项病例对照研究。
Relationship between antioxidant enzyme genotype and activity and kidney function: a case-control study.
作者信息
Crawford Amanda, Fassett Robert G, Coombes Jeff S, Kunde Dale A, Ahuja Kiran D K, Robertson Iain K, Ball Madeleine J, Geraghty Dominic P
机构信息
University of Tasmania, School of Human Life Sciences, Launceston, Tasmania, University of Queensland, School of Medicine, St. Lucia, Australia.
出版信息
Clin Nephrol. 2012 Aug;78(2):135-44. doi: 10.5414/cn107421.
BACKGROUND
Oxidative stress is associated with the progression of chronic kidney disease (CKD). Links between antioxidant enzyme SNPs such as superoxide dismutase (SOD) Ala16Val, glutathione peroxidase (GPx) Pro197Leu and catalase C- 262T and CKD have not been investigated. This study compared antioxidant genotypes and activities of CKD patients with population controls, and determined their relationship to kidney function.
METHODS
CKD patients (n = 230) and controls (n = 224) were screened for the GPx, SOD and catalase SNPs. Plasma and red blood cell (RBC) GPx, RBC SOD and RBC catalase activities, and estimated glomerular filtration rate (eGFR) were measured.
RESULTS
Significantly more CKD patients (n = 5) had the GPx Leu/Leu genotype compared to controls (n = 0), and had lower eGFR (p = 0.054). CKD patients had significantly lower plasma GPx and RBC catalase activities compared to controls, whereas RBC GPx and RBC SOD activities were significantly higher in CKD patients (p < 0.001).
CONCLUSIONS
CKD is associated with reduced plasma GPx and catalase activities and enhanced RBC GPx and SOD activities. Although, genotype frequencies were similar for both groups, lower eGFR was associated with the GPx Leu/ Leu genotype.
背景
氧化应激与慢性肾脏病（CKD）的进展相关。超氧化物歧化酶（SOD）Ala16Val、谷胱甘肽过氧化物酶（GPx）Pro197Leu和过氧化氢酶C-262T等抗氧化酶单核苷酸多态性（SNP）与CKD之间的联系尚未得到研究。本研究比较了CKD患者与正常人群的抗氧化基因型和活性，并确定了它们与肾功能的关系。
方法
对230例CKD患者和224例对照者进行GPx、SOD和过氧化氢酶SNP筛查。检测血浆和红细胞（RBC）中的GPx、RBC SOD和RBC过氧化氢酶活性，以及估算肾小球滤过率（eGFR）。
结果
与对照组（0例）相比，CKD患者中携带GPx Leu/Leu基因型的人数（5例）显著更多，且eGFR更低（p = 0.054）。与对照组相比，CKD患者的血浆GPx和RBC过氧化氢酶活性显著降低，而CKD患者的RBC GPx和RBC SOD活性显著更高（p < 0.001）。
结论
CKD与血浆GPx和过氧化氢酶活性降低以及RBC GPx和SOD活性增强有关。虽然两组的基因型频率相似，但较低的eGFR与GPx Leu/Leu基因型有关。
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