Krabcova Ivana, Studeny Pavel, Jirsova Katerina
Laboratory of the Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, First Faculty of Medicine, General Teaching Hospital, Charles University in Prague, Ke Karlovu 2, 128 08, Prague 2, Czech Republic.
Cell Tissue Bank. 2013 Jun;14(2):325-31. doi: 10.1007/s10561-012-9327-z. Epub 2012 Jul 13.
To assess the quantitative and qualitative parameters of pre-cut posterior corneal lamellae for Descemet membrane endothelial keratoplasty with a stromal rim (DMEK-S) prepared manually in the Ocular Tissue Bank Prague. All 65 successfully prepared pre-cut posterior corneal lamellae provided for grafting during a 2-year period were analyzed retrospectively. The lamellae, consisting of a central zone of endothelium-Descemet membrane surrounded by a supporting peripheral stromal rim, were prepared manually from corneoscleral buttons having an endothelial cell density higher than 2,500 cells/mm(2). The live endothelial cell density, the percentage of dead cells, the hexagonality and the coefficient of variation were assessed before and immediately after preparation as well as after 2 days of organ culture storage at 31 °C. Altogether, the endothelium of 57 lamellae was assessed. Immediately after preparation, the mean live endothelial cell density was 2,835 cells/mm(2) and, on average, 1.8 % of dead cells were found. After 2 days of storage, the cell density decreased significantly to 2,757 cells/mm(2) and the percentage of dead cells to 1.0 %. There was a significant change in the mean hexagonality and the coefficient of variation after lamellar preparation and subsequent storage. The amount of tissue wasted during the preparation was 23 %. The endothelial cell density of posterior corneal lamellae sent for DMEK-S was higher than 2,700 cells/mm(2) in average with a low percentage of dead cells; 65 pre-cut tissues were used for grafting during a 2-year period.
评估在布拉格眼组织库中手工制备的带基质边缘的Descemet膜内皮角膜移植术（DMEK-S）的预切割后角膜板层的定量和定性参数。回顾性分析了在2年期间提供用于移植的所有65个成功制备的预切割后角膜板层。这些板层由内皮-Descemet膜的中央区域和周围的支持性周边基质边缘组成，是从内皮细胞密度高于2500个细胞/mm²的角膜巩膜纽扣中手工制备的。在制备前、制备后立即以及在31°C下器官培养储存2天后，评估活内皮细胞密度、死细胞百分比、六角形度和变异系数。总共评估了57个板层的内皮。制备后立即，平均活内皮细胞密度为2835个细胞/mm²，平均发现1.8%的死细胞。储存2天后，细胞密度显著下降至2757个细胞/mm²，死细胞百分比降至1.0%。板层制备和随后储存后，平均六角形度和变异系数有显著变化。制备过程中浪费的组织量为23%。用于DMEK-S的后角膜板层的内皮细胞密度平均高于2700个细胞/mm²，死细胞百分比低；在2年期间，65个预切割组织用于移植。
