Kellogg Eye Center, University of Michigan, Department of Ophthalmology, Michigan, USA.
Curr Opin Ophthalmol. 2014 Jul;25(4):319-24. doi: 10.1097/ICU.0000000000000060.
Eye-bank preparation of endothelial tissue for keratoplasty continues to evolve. Although eye-bank personnel have become comfortable and competent at Descemet's stripping automated endothelial keratoplasty (DSAEK), tissue preparation and tissue transport, optimization of preparation methods continues. Surgeons and eye-bank personnel should be up to date on the research in the field. As surgeons transit to Descemet's membrane endothelial keratoplasty (DMEK), eye banks have risen to the challenge of preparing tissue. Eye banks are refining their DMEK preparation and transport techniques.
This article covers refinements to DSAEK tissue preparation, innovations to prepare DMEK tissue, and nuances to improve donor cornea tissue quality.
As eye bank-supplied corneal tissue is the main source of tissue for many corneal surgeons, it is critical to stay informed about tissue handling and preparation. Ultimately, the surgeon is responsible for the transplantation, so involvement of clinicians in eye-banking practices and advocacy for pursuing meaningful research in this area will benefit clinical patient outcomes.
眼库对角膜移植用内皮组织的准备工作在不断发展。尽管眼库工作人员已经熟练掌握并胜任了撕囊自动化角膜内皮移植术(DSAEK)、组织准备和组织运输,但仍在不断优化准备方法。外科医生和眼库工作人员应了解该领域的研究进展。随着外科医生向角膜内皮移植术(DMEK)过渡,眼库也已迎接准备组织的挑战。眼库正在改进 DMEK 的准备和运输技术。
本文介绍了 DSAEK 组织准备的改进、用于准备 DMEK 组织的创新技术,以及改善供体角膜组织质量的细微差别。
由于眼库提供的角膜组织是许多角膜外科医生的主要组织来源,因此了解组织处理和准备工作至关重要。最终,外科医生对移植手术负责,因此临床医生参与眼库实践并倡导在该领域开展有意义的研究将有益于临床患者的治疗效果。