Kanavi Mozhgan Rezaei, Javadi Mohammad Ali, Javadi Fatemeh, Chamani Tahereh
Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, No 23, Paydarfard-Boostan 9th St, Pasdaran Avenue, 1666663111, Tehran, Iran,
Cell Tissue Bank. 2014 Sep;15(3):369-72. doi: 10.1007/s10561-013-9392-y. Epub 2013 Aug 10.
To describe the technique and the results of the preparation of pre-cut corneas for Descemet's stripping automated endothelial keratoplasty (DSAEK) during a 3-year period at the Central Eye Bank of Iran (CEBI). The method of preparation of pre-cut corneas from donated whole globes at the CEBI is described and the frequency and percentage of pre-cut corneas prepared for DSAEK, between April 2009 and March 2012, are specified. Moreover, post-operative reports are reviewed for any complaints about using pre-cut tissues for DSAEK. Out of the 1,518 donated whole globes appropriate for DSAEK, 1,478 (97.4 %) pre-cut corneas were successfully prepared. The method of preparation failed in 40 (2.6 %) cases. Based on the eye bank post-operative reports, thickness of pre-cut tissues for DSAEK was deemed unacceptable in only 6 (0.4 %) cases prior to surgery; five of these were too thick and one was too thin. Preparation of pre-cut corneas, for DSAEK from donated whole globes, in the CEBI is a safe and easy method, with very good preservation of endothelial cells after the preparation of the pre-cut corneas and reduced risks from corneal manipulation.
描述伊朗中央眼库（CEBI）在3年期间为Descemet膜剥除自动内皮角膜移植术（DSAEK）制备预切割角膜的技术和结果。描述了CEBI从捐赠的完整眼球制备预切割角膜的方法，并明确了2009年4月至2012年3月期间为DSAEK制备的预切割角膜的频率和百分比。此外，还回顾了术后报告中关于使用DSAEK预切割组织的任何投诉。在1518个适合DSAEK的捐赠完整眼球中，成功制备了1478个（97.4%）预切割角膜。制备方法在40例（2.6%）中失败。根据眼库术后报告，术前仅6例（0.4%）DSAEK预切割组织的厚度被认为不可接受；其中5例太厚，1例太薄。在CEBI，从捐赠的完整眼球制备DSAEK预切割角膜是一种安全简便的方法，预切割角膜制备后内皮细胞保存良好，角膜操作风险降低。
